ISSN:
1617-4623
Keywords:
mRNA stability
;
Translational efficiency
;
Cap
;
Poly(A) tail
;
Higher eukaryotes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary We have investigated whether reporter genes influence cytoplasmic regulation of gene expression in tobacco and Chinese hamster ovary (CHO) cells. Two genes, uidA encoding β-glucuronidase (GUS) from Eseherichia coli and Luc, encoding firefly luciferase (LUC), were used to analyze the ability of a cap, polyadenylated tail, and the 5′- and 3′-untranslated regions (UTR) from tobacco mosaic virus (TMV) to regulate expression. The regulation associated with the 5′ cap structure and the TMV 5′-UTR, both of which enhance translational efficiency, was reporter gene-independent. The poly(A) tail and the TMV 3′-UTR, which is functionally equivalent to a poly(A) tail, increase translational efficiency as well as mRNA stability. The regulation associated with these 3′ ends was highly reporter gene-dependent; their effect on GUS expression was almost an order of magnitude greater than that on LUC expression. In tobacco, the tenfold reporter gene effect on poly(A) tail or TMV 3′-UTR function could not be explained by a differential impact on mRNA stability; GUS and LUC mRNA half-life increased only twofold when either the poly(A) tail or TMV 3′-UTR was present. In CHO cells, however, GUS mRNA was stabilized to a greater extent by a poly(A) tail or the TMV 3′-UTR than was LUC mRNA.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00282474
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