ISSN:
1432-0983
Keywords:
Glucoamylase
;
Gene cloning
;
Hormoconis resinae
;
Saccharomyces cerevisiae
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A cDNA coding for glucoamylase P of Hormoconis resinae was cloned using a synthetic oligonucleotide probe coding for a peptide fragment of the purified enzyme and polyclonal anti-glucoamylase antibodies. Nucleotide-sequence analysis revealed an open reading frame of 1848 base pairs coding for a protein of 616 amino-acid residues. Comparison with other fungal glucoamylase amino-acid sequences showed homologies of 37–48%. The glucoamylase cDNA, when introduced into Saccharomyces cerevisiae under the control of the yeast ADC1 promoter, directed the secretion of active glucoamylase P into the growth medium.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00324663
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