ISSN:
0148-7280
Keywords:
Chlamydomonas
;
sulfhydryl groups
;
plasma membrane
;
adhesion
;
receptor pathching
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
Reagents that block or cross-link sulfhydryl (-SH) groups and those that reduce disulfide (S-S) bonds have been tested for their effects on mating in Chlamydomonas reinhardii. Wild-type (wt) gametes of mating type + (mt+) and mt-, and a fusion-defective mt- mutant, gam-11, were studied. Differential sensitivities of mt+ vs mt- and of wt mt- vs gam-11 mt- were analyzed. Concentrations of reagents that did not disrupt flagellar agglutination, the first stage of the mating reaction, were generally used. Pretreatment of mt+ gametes with the membrane permeable -SH reducing agent dithiothreitol (DTT) inhibits flagellar sexual signaling at concentrations that do not inhibit any part of the mating reaction of mt- gametes. Wt mt- is more sensitive than wt mt+ to inhibition by low concentrations of p-chloromercuribenzoate sulfonate (pCMBS), an organic mercurial. The membrane-impermeable reducing agent, reduced glutathione (GSH), also preferentially inhibits wt mt-. Gam-11 mt-, a fusion-defective mutant, which has been used to study the sensitivity of the adhesion of the plasma membrane-associated mating structures, is less sensitive to GSH and pCMBS inhibition that is wt mt-. DDT and pCMBS cause an increase in mating structure adhesion in pretreated gam-11. The differential inhibition of pair and group formation during gam-11 × wt mt+ matings has suggested a possible mechanism for mating structure adhesion.
Additional Material:
5 Tab.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mrd.1120120205
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