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Surface Anionic Groups in Symbiote-Bearing and Symbiote-Free Strains of Crithidia deanei (1984)

ODA, LEILA M. ; ALVIANO, CELUTA S. ; FILHO, FERNANDO C. SILVA ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

The @journal of eukaryotic microbiology 31 (1984), S. 0

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ISSN: 1550-7408

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: The surface anionic groups of symbiote-bearing and symbiote-free strains of Crithidia deanei were compared by determining cellular electrophoretic mobility, by ultrastructural cytochemistry, and by identification of sialic acids by thin-layer and gasliquid chromatography. Symbiote-free Crithidia deanei has a highly negative surface charge (-0.9984 μmċs-1ċ V-1ċ cm), which is slightly reduced (-0.8527 μmċs-1ċ V-1ċ cm) by the presence of the endosymbiote. Treatment of both strains of C. deanei with neuraminidase decreased significantly the electrophoretic mobility of cells toward the cathodic pole, indicating the existence of exposed sialic acid residues responsible for the negative charge on the protozoan cell surface. Thin-layer and gas-liquid chromatography showed that N-glycolyl- and N-acetylneuraminic acids were present in both strains of C. deanei.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1550-7408.1984.tb04301.x

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Surface charge and surface carbohydrates of cryptobia salmositica virulent and avirulent forms and of C. bullocki (Kinetoplastida: Cryptobiidae) (1997)

Vommaro, Rossiane C. ; Attias, Ma´rcia ; Filho, Fernando C. Silva ; [et al.]

Springer

Parasitology research 83 (1997), S. 698-705

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ISSN: 1432-1955

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract The surface charge and surface carbohydrate residues of the virulent (freshly isolated from the fish blood) and avirulent forms (from culture) of Cryptobia salmositica and one strain of C. bullocki were studied. Measurements of the zeta potential of parasites showed that C. bullocki and the virulent form of C. salmositica had a net negative surface charge of about −15 mV, whereas the attenuated form of C. salmositica showed a surface charge of −7.9 mV. Enzymatic treatments of parasites with neuraminidase, trypsin, or phospholipase C indicated the presence of sialic acid residues, phosphate groups, and protein glycoconjugates as components of the Cryptobia surface that accounted for their surface charge. Residues of α-D-man, α- and β-D-gal, α-D-galNAc, α-L-fuc, and D-glcNAc could be detected on the surface of all parasites by specific fluorescein isothiocyanate (FITC)- and colloidal gold-labeled lectins. The cell surface of the avirulent form of C. salmositica showed the strongest reactivity to almost all lectins tested. A remarkable binding pattern of lectins in the anterior region of parasites was observed.