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  • 1
    Electronic Resource
    Electronic Resource
    GRP/Bombesin-Immunoreactive Sacciform Gland Cells in the Epidermis of the Clingfish, Lepadogaster candollei (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 547 (1988), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1988.tb23920.x
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  • 2
    Electronic Resource
    Electronic Resource
    Patterns of enzyme activities in the gills of the catfish Heteropneustes fossilis (Bloch) exposed to the anionactive detergent Na-alkyl-benzenesulphonate (LAS) (1985)
    Springer
    Histochemistry and cell biology 82 (1985), S. 341-343 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effect of sodium-alkyl-benzenesulphonate (LAS) on the activity of the respiratory enzymes of the gills of Heteropneustes fossilis (Bloch) was investigated. After 48 h exposure, the main injury to gills was the progressive separation of the lamellae from their vascular components. The enzymes of the aerobic part of the metabolism showed a decrease in activity, whereas the activity of lactate dehydrogenase was strongly increased, thus indicating that LAS has a high potential to interfere with aerobic mechanisms; however, the mode of action of it has yet to be clearly defined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00494062
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  • 3
    Electronic Resource
    Electronic Resource
    Lectin-binding pattern on the surface epidermis of Ambystoma tigrinum larvae (1987)
    Springer
    Histochemistry and cell biology 87 (1987), S. 431-438 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The surface epidermis of Ambystoma tigrinum larvae was examined at the light- and electron-microscope levels using five different lectin conjugates as probes for the detection of sugar residues on the cell membranes. Concanavalin A (Con-A), wheat-germ agglutinin (WGA), Ricinus communis agglutinin I (RCA-I), Dolichos biflorus agglutinin and soybean agglutinin (SBA) conjugates clearly labelled the surface cells, especially their apical surfaces. At electron microscopy, the labelling on plasma membranes was found to exhibit regional differences. Among the lectins tested WGA displayed a particularly characteristic binding pattern. WGA also bound to basolateral cell surfaces, including the tight-junction zone wich was also stained by the RCA-I conjugate. The different labelling intensity and staining patterns obtained with the conjugates indicated the polarity of the cell surfaces. It is also assumed that the WGA staining of the basolateral membranes and intercellular spaces reflected transcellular transport, which is facilitated by acidic glycoconjugates. Other functional aspects of the polarized distribution of the lectin conjugates were also correlated with the receptor sites of certain sugar residues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00496814
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  • 4
    Electronic Resource
    Electronic Resource
    Serotonin-like immunoreactivity in the epidermal club cells of teleost fishes (1990)
    Springer
    Histochemistry and cell biology 93 (1990), S. 355-357 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The present immunocytochemical study concerns the distribution of serotonin in the epidermis of three species of teleost fish. Serotonin-like immunoreactivity was found in the club cells of Heteropneustes fossilis and Carapus acus but not in those from the sea eel Conger conger. This study is the first immunocytochemical identification of serotonin in the club cells of teleost epidermis. By comparing data from the literature (Zaccone et al. 1986, 1987, 1988) regarding the occurrence of serotonin and GRP/bombesin in the exocrine sacciform gland cells of piscine skin, it is worthy mentioning here that the serotonin contained in the club cells of the species studied may have the ability to affect the pheromonal or other possible functions of these cells. The presence of serotonin in these systems has been correlated with the capacity of the exocrine glands of fish skin to secrete, ectopically, amine messengers in contrast to those produced eutopically i.e. in the neuron-paraneuron system in some vertebrates (Fujita et al. 1988).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315850
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  • 5
    Electronic Resource
    Electronic Resource
    Ultrastructural demonstration of alkaline phosphatase (ALP) and K+-p-nitrophenyl phosphatase (K+-p-NPPase) in the epidermal ionocytes of Blennius sanguinolentus (1984)
    Springer
    Histochemistry and cell biology 81 (1984), S. 47-53 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cytochemical techniques were used for the light and electron microscopical localization of alkaline phosphatase and potassium-dependent nitrophenyl phosphatase in the epidermal ionocytes of the Teleost Blennius sanguinolentus. The heavier deposition of the reaction products obtained with the different media was shown in the cytoplasmic surface of the labyrinth tubules, the apical vesicles and in intimate association with plasmic membranes. Both plasma membranes and intracellular activities are affected by the addition of specific inhibitors l-p-bromotetramisole oxalate and ouabain) to both complete and control media. The significance of the cytoplasmic localization of both the two enzymes is discussed with reference to current models of transepithelial ion transportation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495400
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  • 6
    Electronic Resource
    Electronic Resource
    Enzyme cytochemical and immunocytochemical studies of flask cells in the amphibian epidermis (1986)
    Springer
    Histochemistry and cell biology 84 (1986), S. 5-9 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The localization of oxidoreductases and transport enzymes in flask cells of the amphibian epidermis was studied at the light-microscopic level. In these cells, the deposition of cytochemical reaction products was very similar to that found in fish epidermal ionocytes, thus demonstrating histochemical similarities between these two types of cells. The present histochemical results revealed high levels of activity of alkaline phosphatase (ALPase), potassium-dependent nitrophenylphosphatase (K+-p-NPPase) and carbonic-anhydrase isozymes (CA-I and CA-II) in the apical region of the flask cells, indicating that enzyme zonation may be the main site of the ion pumping.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493412
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  • 7
    Electronic Resource
    Electronic Resource
    Serotonin-like immunoreactive cells in the pulmonary epithelium of ancient fish species (1989)
    Springer
    Histochemistry and cell biology 92 (1989), S. 61-63 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The pulmonary mucosa of three species of ancient fish was studied immunohistochemically to show the distribution of serotonin, regarded as the main monoamine of mammalian bronchopulmonary paraneurons. Serotonin-like immunoreactive cells, dispersed through the airway epithelium as single cells, were found in all the fish species studied. They are presumably equivalent to the neuroendocrine cells reported in the lungs of mammalian and submammalian vertebrates. However, the precise role and the function of these cells remain unknown. Since the species studied belong to the most primitive extant groups of ancient fish, the present investigation suggests that serotonin is widely distributed in the lungs of the vertebrates. Several peptides, known to be specific cytochemical markers for the identification of the pulmonary neuroendocrine cells of mammals, are being investigated in the lungs of the fish species studied. They may help to trace the phylogeny of the pulmonary neuroendocrine cell system and to elucidate its function in lower vertebrates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495017
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  • 8
    Electronic Resource
    Electronic Resource
    Localization of calmodulin positive immunoreactivity in the surface epidermis of the brown trout, Salmo trutta (1989)
    Springer
    Histochemistry and cell biology 91 (1989), S. 13-16 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Calmodulin is a Ca2+-dependent modulatory protein which is required in the general regulation of a large number of key processes of cellular metabolism. In the present study, the localization of calmodulin positive immunoreactivity in the epidermis of the brown trout, Salmo trutta was investigated using a specific mouse monoclonal antibody to calmodulin of IgG2 class. The immunoreaction was found only in the superficial epithelial cells that constitute the main histological site for the production of calmodulin positive substances. Because of its distribution, this protein might have a physiological significance in the activation of the microvillar skeleton and in the control of the permeability of the skin epithelium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00501904
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  • 9
    Electronic Resource
    Electronic Resource
    Distribution of immunoreactive Tamm-Horsfall protein in various species in the vertebrate classes (1993)
    Springer
    Cell & tissue research 274 (1993), S. 15-19 
    Details
    ISSN: 1432-0878
    Keywords: Tamm-Horsfall protein ; Skin ; Gills ; Kidney ; Vertebrata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A sheep antibody to human Tamm-Horsfall protein, the major protein in normal urine, was used in an immunohistological study of organs of 48 species of vertebrate animals, representing the classes Mammalia, Aves, Reptilia, Amphibia, Osteichthyes and Chondrichthyes. Immunoreacvity was shown in the thick limb of the loop of Henle in the kidney of mammals, but there was no reactivity with tissues of birds or reptiles. Superficial layers of the skin of several amphibians and fish, superficial layers of the oral mucosa and gills of fish, and the distal tubules of the kidney of some amphibians, reacted with the antibody. Immunoreactivity with mammalian kidney was removed by passage of the antibody down an immunoadsorption column coated with human Tamm-Horsfall protein, and amphibian immunoreactivity was removed by incubation of the antibody with material prepared from frogs in the same way as Tamm-Horsfall protein. These findings suggest that immunoreactive Tamm-Horsfall protein appeared early in vertebrate phylogeny, initially in skin and gills and later in kidney, and that although conserved in evolution, it shows antigenic differences between amphibians and mammals. Its distribution is consistent with the hypothesis that it acts as a waterproofing agent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00327980
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  • 10
    Electronic Resource
    Electronic Resource
    5-Hydroxytryptamine immunoreactivity in the epidermal sacciform gland cells of the clingfish Lepadogaster candollei Risso (1986)
    Springer
    Cell & tissue research 246 (1986), S. 679-682 
    Details
    ISSN: 1432-0878
    Keywords: Fish skin ; Sacciform glandular cells ; Immunocytochemistry ; 5-Hydroxytryptamine ; Lepadogaster candollei
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Serotonin has been demonstrated in the epidermal sacciform glandular cells of the clingfish Lepadogaster candollei by use of immunocytochemistry. Serotonin immunoreactivity is found both in the peripheral cytoplasm of the glandular cells and their luminal secretion. The presence of serotonin in the sacciform glandular cells parallels that located by both biochemical and immunocytochemical procedures in the cutaneous glands of many amphibian species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215211
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