Publication Date:
2009-08-28
Description:
Cyanobacteria of the Synechococcus and Prochlorococcus genera are important contributors to photosynthetic productivity in the open oceans. Recently, core photosystem II (PSII) genes were identified in cyanophages and proposed to function in photosynthesis and in increasing viral fitness by supplementing the host production of these proteins. Here we show evidence for the presence of photosystem I (PSI) genes in the genomes of viruses that infect these marine cyanobacteria, using pre-existing metagenomic data from the global ocean sampling expedition as well as from viral biomes. The seven cyanobacterial core PSI genes identified in this study, psaA, B, C, D, E, K and a unique J and F fusion, form a cluster in cyanophage genomes, suggestive of selection for a distinct function in the virus life cycle. The existence of this PSI cluster was confirmed with overlapping and long polymerase chain reaction on environmental DNA from the Northern Line Islands. Potentially, the seven proteins encoded by the viral genes are sufficient to form an intact monomeric PSI complex. Projection of viral predicted peptides on the cyanobacterial PSI crystal structure suggested that the viral-PSI components might provide a unique way of funnelling reducing power from respiratory and other electron transfer chains to the PSI.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4605144/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4605144/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sharon, Itai -- Alperovitch, Ariella -- Rohwer, Forest -- Haynes, Matthew -- Glaser, Fabian -- Atamna-Ismaeel, Nof -- Pinter, Ron Y -- Partensky, Frederic -- Koonin, Eugene V -- Wolf, Yuri I -- Nelson, Nathan -- Beja, Oded -- Z99 LM999999/Intramural NIH HHS/ -- England -- Nature. 2009 Sep 10;461(7261):258-62. doi: 10.1038/nature08284. Epub 2009 Aug 26.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Faculty of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19710652" target="_blank"〉PubMed〈/a〉
Keywords:
Adhesins, Bacterial/chemistry/genetics
;
Amino Acid Sequence
;
Bacteriophages/*genetics/metabolism
;
Biodiversity
;
Genes, Bacterial/genetics
;
Genes, Viral/*genetics
;
Genome, Bacterial/genetics
;
Genome, Viral/*genetics
;
Geography
;
Lipoproteins/chemistry/genetics
;
Models, Molecular
;
Molecular Sequence Data
;
Oceans and Seas
;
Open Reading Frames/genetics
;
Oxidation-Reduction
;
Photosynthesis/genetics
;
Photosystem I Protein Complex/chemistry/*genetics
;
Phylogeny
;
Polymerase Chain Reaction
;
Prochlorococcus/*virology
;
Protein Conformation
;
Seawater/*microbiology
;
Synechococcus/*virology
;
Viral Proteins/chemistry/genetics/metabolism
;
Water Microbiology
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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