Publication Date:
1985-04-26
Description:
A multiplicity of cytochromes P-450 is responsible for the detoxification and activation of xenobiotics such as drugs and carcinogens. Individual differences in sensitivity to these agents may reside in the cytochrome P-450 phenotype. A monoclonal antibody-directed radioimmunoassay was developed that detects epitope-specific cytochromes P-450 in human placentas and peripheral lymphocytes. Placentas from women who smoked cigarettes contained greater amounts of cytochrome P-450 with the monoclonal antibody-specific epitope than placentas from nonsmokers. The amount of this cytochrome P-450 in human peripheral lymphocytes increased after treatment of the mitogenized lymphocytes with the cytochrome P-450 inducer benz[a]anthracene.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Song, B J -- Gelboin, H V -- Park, S S -- Tsokos, G C -- Friedman, F K -- New York, N.Y. -- Science. 1985 Apr 26;228(4698):490-2.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2580351" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antibodies, Monoclonal
;
Antibody Specificity
;
Aryl Hydrocarbon Hydroxylases/metabolism
;
Benz(a)Anthracenes/pharmacology
;
Cytochrome P-450 Enzyme System/*analysis/immunology
;
Enzyme Induction/drug effects
;
Epitopes/immunology
;
Humans
;
Lymphocytes/*enzymology
;
Methylcholanthrene/pharmacology
;
Microsomes/enzymology
;
Microsomes, Liver/enzymology
;
Placenta/*enzymology
;
Radioimmunoassay/methods
;
Rats
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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