Publication Date:
1997-07-18
Description:
Proteins of the Bcl-2 family are intracellular membrane-associated proteins that regulate programmed cell death (apoptosis) either positively or negatively by as yet unknown mechanisms. Bax, a pro-apoptotic member of the Bcl-2 family, was shown to form channels in lipid membranes. Bax triggered the release of liposome-encapsulated carboxyfluorescein at both neutral and acidic pH. At physiological pH, release could be blocked by Bcl-2. Bcl-2, in contrast, triggered carboxyfluorescein release at acidic pH only. In planar lipid bilayers, Bax formed pH- and voltage-dependent ion-conducting channels. Thus, the pro-apoptotic effects of Bax may be elicited through an intrinsic pore-forming activity that can be antagonized by Bcl-2.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Antonsson, B -- Conti, F -- Ciavatta, A -- Montessuit, S -- Lewis, S -- Martinou, I -- Bernasconi, L -- Bernard, A -- Mermod, J J -- Mazzei, G -- Maundrell, K -- Gambale, F -- Sadoul, R -- Martinou, J C -- New York, N.Y. -- Science. 1997 Jul 18;277(5324):370-2.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Geneva Biomedical Research Institute, Glaxo Wellcome R&D S. A., 1288 Plan les Ouates, Geneva, Switzerland. beau6063@ggr.co.uk〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9219694" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Apoptosis
;
Cell Membrane Permeability
;
Cells, Cultured
;
Erythrocytes/cytology
;
Fluoresceins/metabolism
;
Hemolysis
;
Humans
;
Hydrogen-Ion Concentration
;
Ion Channels/*physiology
;
Lipid Bilayers
;
Liposomes
;
Membrane Potentials
;
Neurons/cytology
;
Patch-Clamp Techniques
;
Proto-Oncogene Proteins/pharmacology/*physiology
;
Proto-Oncogene Proteins c-bcl-2/antagonists & inhibitors/pharmacology/*physiology
;
Sheep
;
Sympathetic Nervous System/cytology
;
bcl-2-Associated X Protein
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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