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  • 1
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    Etudes cytologiques de choux tétraploïdes (Brassica oleracea L. ssp. acephala) obtenus à partir de lignées diploïdes après traitement à la colchicine (1989)
    EDP Sciences
    Details
    Publication Date: 1989-01-01
    Print ISSN: 0249-5627
    Electronic ISSN: 1297-9643
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by EDP Sciences
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  • 2
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    Assessment of Gene Flow Between Gossypium hirsutum and G. herbaceum: Evidence of Unreduced Gametes in the Diploid Progenitor (2017)
    Genetics Society of America (GSA)
    Details
    Publication Date: 2017-07-06
    Description: In the framework of a gene flow assessment, we investigated the natural hybridization rate between Gossypium hirsutum (AADD genome) and G. herbaceum (AA genome). The latter species, a diploid progenitor of G. hirsutum , is spontaneously present in South Africa. Reciprocal crosses were performed without emasculation between G. herbaceum and G. hirsutum . Neither examination of the morphological characteristics nor flow cytometry analysis of the 335 plants resulting from the G. hirsutum x G. herbaceum cross showed any hybrid features. Of the 148 plants produced from the G. herbaceum x G. hirsutum cross, three showed a hybrid phenotype, and their hybrid status was confirmed by SSR markers. Analysis of DNA content by flow cytometry and morphological traits clearly showed that two of these plants were triploid (AAD). The third plant had a flow cytometry DNA content slightly higher than G. hirsutum . In addition, its morphological characteristics (plant architecture, presence and size of petal spots, leaf shape) led us to conclude that this plant was AAAD thus resulting from fertilization with an unreduced AA gamete of the female G. herbaceum parent. Fluorescent In Situ Hybridization (FISH) and meiotic behavior confirmed this hypothesis. To the best of our knowledge, this is the first description of such gametes in G. herbaceum , and it opens new avenues in breeding programs. Furthermore, this plant material could provide a useful tool for studying the expression of genes duplicated in the A and D cotton genome.
    Electronic ISSN: 2160-1836
    Topics: Biology
    Published by Genetics Society of America (GSA)
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  • 3
    Electronic Resource
    Electronic Resource
    Wide mapping of a T-DNA insertion site in oilseed rape using bulk segregant analysis and comparative mapping (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 116 (1997), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Doubled haploid oilseed rape lines segregating for a transgene inducing herbicide resistance (bar gene) were investigated for the wide mapping of the T-DNA insertion site. Bulk segregant analysis using presence/absence and intensity polymorphisms between the bulks, as well as comparative mapping with a linkage group deriving from another cross, led to the identification of 11 random amplified polymorphic DNA (RAPD) markers tightly or loosely linked to the bar gene. Ten RAPD loci out of 11 were located on the same side of the bar locus, strongly suggesting that the T-DNA integrated in a telomeric or subtelomeric position. The eleventh RAPD marker exhibited a strong segregation distortion, which could be the result of a heteroduplex formation. Comparison of the linkage groups obtained from the two crosses showed different recombination rates between markers, possibly reflecting differences in parental genetic backgrounds. Consequences and potential applications in transgene dispersal safety assessment studies are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1997.tb02189.x
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  • 4
    Electronic Resource
    Electronic Resource
    Genetic analysis and nomenclature for seven isozyme systems in Brassica nigra, B. oleracea and B. campestris (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 114 (1995), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: General criteria for the assignment of names to enzyme systems, regions of activity, isozyme loci and allozymes have been lacking in crucifer species. This paper proposes a standard nomenclature for seven isozyme systems in the three diploid species of U's triangle: Brassica nigra, B. oleracea and B. campestris. Gel/electrode buffers, which provided the best resolution for seven isozyme systems, acid phosphatase (APS), aconitase (ACO), leucine aminopeptidase (LAP), 6-phosphogluconate dehydrogenase (6 PGD), phosphoglucoisomerase (PGI), phosphoglucomutase (PGM), and triosephosphate isomerase (TPI), were proposed as standards. Isozyme genetic analysis was determined for B. oleracea and B. campestris from previous studies and by segregation of selfed progenies of heterozygous B. nigra plants. Several populations were studied and 148 allozymes at the 18 loci observed were described for the three species. Their relative mobility was studied using a pure line of oilseed rape as reference. The comparison of the different alleles within and between the species is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1995.tb00839.x
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  • 5
    Electronic Resource
    Electronic Resource
    A strict diploid-like pairing regime is associated with tetrasomic segregation in induced autotetraploids of kale (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 121 (2002), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Fertility and reproductive stability in polyploids depend on the precise control of chromosome pairing at meiosis. In this study, meiotic pairing configurations are related to isozyme segregation ratios in F1 hybrids between colchicine-induced autotetraploids of kale. An immediate diploid-like pairing regime is associated with tetrasomic segregation. Although the mechanisms driving such a behaviour have yet to be identified, pairing preferences are not responsible for the cytological diploidization of autotetraploid kales.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2002.00672.x
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  • 6
    Electronic Resource
    Electronic Resource
    Characterization of Brassica nigra chromosomes and of blackleg resistance in B. napus–B. nigra addition lines (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 115 (1996), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Brassica napus-B. nigra addition lines were previously created using the variety ‘Darmor’ as the oilseed rape genetic background. Two isozyme loci and 46 RAPD markers were added on five different B. nigra chromosomes. The oilseed rape variety used was highly susceptible to blackleg at the cotyledon stage and only the addition of chromosome 4 gave the same level of blackleg resistance as B. nigra. This resistance was efficient whatever the isolates used. A significant effect on the development of stem canker under field conditions was observed only for the line carrying chromosome 4 which was more resistant than the susceptible control. The potential effects of two other chromosomes have to be confirmed. F1 hybrids obtained by crosses between two highly susceptible lines and the monosomic addition line carrying chromosome 4 were examined under field conditions. No effect of the oilseed rape genetic background on the expression of resistance was detected. The introduction of this resistance and mapping of the gene(s) into oilseed rape varieties are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1996.tb00884.x
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  • 7
    Electronic Resource
    Electronic Resource
    Electrophoretic analysis of natural populations of Leptosphaeria maculans directly from leaf lesions (1997)
    Oxford, U.K. and Cambridge, USA : Blackwell Science Ltd
    Plant pathology 46 (1997), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: On oilseed rape, 207 leaf lesions attributed to Leptosphaeria maculans were classified as typical or atypical. Starch gel electrophoresis of glucose phosphate isomerase (GPI) performed on extracts of 229 leaf lesions comprising the 207 with L. maculans symptoms and 22 with Pseudocercosporella capsellae symptoms, yielded four different electrophoretic patterns of alloenzymes designated ET1 to ET4. In addition to ET1 and ET2, characteristic respectively of A- (highly virulent) and B- (weakly virulent) group isolates of L. maculans, the previously undescribed ET3 allozyme was recovered from a few typical and atypical L. maculans leaf lesions. The fastest ET4 allozyme was specific to P. capsellae. All but two typical leaf lesions produced the ET1 allozyme, whereas atypical lesions produced one of the three L. maculans allozymes. Occasionally a mixture of two allozymes was recovered from a same-leaf lesion. GPI electrophoresis performed directly on leaf lesions proved a useful and reliable method to identify L. maculans, and to differentiate between L. maculans and P. capsellae. This method of discrimination enabled deductions, from 377 leaf lesions analysed, about the structure of L. maculans populations on different oilseed rape varieties.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3059.1997.d01-209.x
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  • 8
    Electronic Resource
    Electronic Resource
    Assessment of interspecific hybridization between transgenic oilseed rape and wild radish under normal agronomic conditions (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 1233-1239 
    Details
    ISSN: 1432-2242
    Keywords: Key words Transgenic oilseed rape ; Wild radish ; Hybridization rate ; Agronomic conditions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  In order to assess the hybridization rate between oilseed rape and wild radish under normal agronomic conditions, three 1-ha field experiments were performed. In each case, wild radish plants were transplanted at different densities in the middle, the border, or the margin of the herbicide-tolerant oilseed rape field. Among the 189084 seedlings obtained from seeds harvested on wild radish plants, only one herbicide-tolerant interspecific hybrid (RrRrAC, 2n = 37) was characterized from seeds harvested on an isolated plant growing in the margin of the field. Thus, for the wild radish total harvest, with a 95% confidence limit, the frequency of interspecific hybrids was assessed to range from 10–7 to 3.10–5. Interspecific hybrids were detected in all cases among the smallest seeds with a diameter less than 1.6 mm harvested on oilseed rape, but the highest frequency was obtained from oilseed rape close to wild radish plants growing as clusters in the border or the margin of the field. Most hybrids had the expected triploid genomic structure (ACRr, 2n = 28) except for four amphidiploids (AACCRrRr, 2n = 56) and one hybrid from a wild radish unreduced gamete (ACRrRr, 2n = 37). Among the 73847 seedlings observed on the oilseed rape total harvest, the frequency of interspecific hybrids was assessed to range from 2.10–5to 5.10–4, with a 95% confidence limit. The results are discussed with regard to the type of oilseed rape variety used and the characteristics of the interspecific hybrids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051429
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  • 9
    Electronic Resource
    Electronic Resource
    Selection of stable Brassica napus-B. juncea recombinant lines resistant to blackleg (Leptosphaeria maculans). 1. Identification of molecular markers, chromosomal and genomic origin of the introgression (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 1104-1111 
    Details
    ISSN: 1432-2242
    Keywords: Key words Brassica napus ; B. juncea ; B. nigra addition lines ; Blackleg resistance ; RAPD markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A scheme of selection combining selfing and backcross was applied to a B. napus line with the blackleg resistance from B. juncea in order to transfer this resistance to a winter oilseed rape variety. Cytogenetic analyses combined with cotyledon blackleg resistance tests at each generation allowed us to obtain a recombinant line showing regular meiotic behavior. The resistance is monogenic and is highly efficient under field conditions. Four-hundred RAPD primers were tested on two segregating populations by bulk segregant analysis. Three markers totally linked to the introgression were identified. The analysis of these markers on both sets of B. napus-B. nigra and B. oleracea-B. nigra addition lines revealed that they are not located on the B4 chromosome of B. nigra, which has already been shown to carry a blackleg resistance gene, but rather on the B8 chromosome. We confirmed that the resistance gene is carried by the B genome of B. juncea. Based on these data, two hypotheses, one involving chromosome rearrangements between the two B genomes of B. nigra and B. juncea, and the other based on a more probable digenic control of the resistance within B. juncea, are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050669
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  • 10
    Electronic Resource
    Electronic Resource
    Linkage between an isozyme marker and a restorer gene in radish cytoplasmic male sterility of rapeseed (Brassica napus L.) (1992)
    Springer
    Theoretical and applied genetics 85 (1992), S. 222-228 
    Details
    ISSN: 1432-2242
    Keywords: Brassica napus ; Raphanus sativus ; Cytoplasmic male sterility ; Restorer ; Isozyme
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Co-segregation studies of isozyme markers and male fertility restoration showed that a restorer gene from radish was introduced into rapeseed along with an isozyme marker (Pgi-2). The radish chromosome segment carrying these genes was introgressed into rapeseed through homoeologous recombination, substituting for some of the rapeseed alleles. By crossing heterozygous restored plants to male-sterile lines and to maintainers, tight linkage was found between the restorer gene and the marker. The recombination fraction was estimated at 0.25 ± 0.02%. Although few restored plants lacked the radish isozyme marker, it was still possible to distinguish male-fertile from male-sterile plants by their PGI-2 patterns. Furthermore, homozygous and heterozygous restored plants could be separated by specific PGI-2 phenotypes. Thus, the Pgi-2 marker is now currently used in restorer breeding programs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222863
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