ALBERT

Notes: Five pasture treatments were applied to three semi-natural hill grassland communities. The pasture treatments were: (1) controlled grazing, (2) controlled grazing + lime, (3) controlled grazing + lime + phosphate, (4) controlled grazing + lime + phosphate + oversown white clover and (5) controlled grazing + lime + phosphate + oversown white clover + oversown perennial ryegrass. The communities were dominated by Agrostis/Festuca (site 1), Molinia (site 2) and Nardus (site 3). The Nardus at site 3 was substantially reduced by herbicide before treatments were applied. All treatments were grazed simultaneously by mature wether sheep on three occasions each year to a residual herbage mass of 560 kg DM ha−1. There were two grazing periods, each of 4 weeks duration, beginning in mid-May and mid-July respectively and a further grazing period of 3 weeks duration beginning in mid-October. The number of sheep grazing days and liveweight gain of sheep were recorded during each grazing period for 13 years at each site. Organic matter intake (OMI) and digestibility (OMD) of ingested herbage were measured in years 2, 5 and 13.Mean daily OMI per head ranged from 1290 g for treatment 1 to over 1400 g for treatments 4 and 5. OMI values were higher for the Agrostis/Festuca site (mean OMI 1450 g) than for the Molinia and Nardus sites (1310 g and 1370 g respectively) largely owing to differences in the values for treatments 1, 2 and 3. OMI values for each treatment decreased by around 300 g between May and October.Mean OMD values from treatments 1, 2 and 3 were higher for the Agrostis/Festuca site (0·66) than for the Molinia and Nardus sites (0·63 and 0·64 respectively). The OMD values for treatments 1, 2 and 3 at the Molinia and Nardus sites declined by between 0·05 and 0·02 between years 2 and 13 and also showed the greatest decrease between May and October (0·08). OMD values for treatments 4 and 5 (0·67 and 0·69 respectively) were higher than for treatments 1, 2 and 3 at all sites and levels were maintained over 13 years.Individual liveweight gains of sheep increased from treatment 1 (−18 g d−1) to treatment 5 (82 g d−1), but they decreased over time on all treatments.The Agrostis/Festuca site gave 17% more annual sheep grazing days than the Molinia site and 33% more than the Nardus site. The mean number of annual sheep grazing days for each treatment ranged from 2250 for treatment 1 to 3640 for treatment 5. Annual sheep grazing days increased over 13 years by between 35 and 45% for treatment 1 and 55 and 70% for treatment 5.The results are described in relation to the changes in pasture composition and herbage accumulation.

Notes: Five pasture treatments: (1) controlled grazing, (2) controlled grazing + lime, (3) controlled grazing + lime + phosphate, (4) controlled grazing + lime + phosphate + oversown white clover and (5) controlled grazing + lime + phosphate + oversown white clover + oversown perennial ryegrass were applied to three semi-natural hill grassland communities. The communities were those dominated by Agrostis/Festuca, Molinia and Nardus. The proportion of Nardus at the Nardus-dominant site was substantially reduced by herbicide before treatments were applied. All treatments were grazed simultaneously by mature wether sheep on three occasions each year. There were two grazing periods each of 4 weeks duration between mid-May and mid-August with a further grazing period of 3 weeks starting mid-October. During each grazing period stock numbers were set so that a residual herbage mass of 560 kg DM ha −1 remained at the end of the grazing period. Measurements of net herbage accumulation (NHA) were made annually over a period of 13 years at each site. The green:dead ratio of grasses, species composition of the pasture and the pH of the soil were measured at intervals during the experiment.Estimates of mean annual NHA ranged from 3860 kg DM ha−1 for treatment 1 (controlled grazing) to 5170 kg DM ha−1 for treatment 5 (oversown white clover and perennial ryegrass). The application of lime and phosphate increased annual NHA by 300–350 kg DM ha−1 with a further increase of around 400 kg DM ha−1 when white clover was sown. Increases in NHA between year 1 and year 13 ranged from 30% for treatment 1 to around 55% for treatment 5. Although there was no difference in the mean NHA between sites, the herbage from the Agrostis/Festuca site contained a higher proportion of green grass and white clover than that from the other sites. The highest levels of green grass and white clover were found on the oversown treatments at each site.The grazing pressure exerted produced relatively little change in the botanical composition at the Agrostis/Festuca site. At the Molinia-dominant site the Molinia was largely replaced by Nardus during the first 6 years and Nardus also increased in cover at the Nardus site. Application of lime and phosphate generally increased the proportion of Poa pratensis, Festuca rubra and Agrotis tenuis but did not halt the spread of Nardus at the Molinia and Nardus sites. White clover and perennial ryegrass were successfully introduced by oversowing and proportions remained high throughout the 13 years. The cover by bryophyte spp. increased at all sites with the greatest increases occurring in each case on the less comprehensive pasture treatments.These results suggest that on Agrostis/Festuca-dominant pastures herbage biomass production can be increased with relatively low-cost pasture treatments while maintaining Species diversity. However, Nardus and Molinia dominant pastures are likely to require more comprehensive pasture treatments involving sown grasses and white clover to provide herbage of acceptable quality and avoid an increase in Nardus and bryophytes in the sward. With a regime of episodic summer grazing and the addition of fertilizers oversown pastures can be maintained over long periods of time.

Notes: [Auszug] The mutagenicity of O6meG lesions was analysed using a forward mutation assay (Fig. 1), which makes use of the single-stranded bacteriophage f1/PBR 322 chimaera designated R208 17. The pBR322 insert of R208 carries trwo selectable genes, Ampr and Tetr , which confer resistance to the antibiotics ...

Notes: [Auszug] Table 1. COUNTS OF FREE BACTERIA IN LAMBS WITH AND WITHOUT CILIATE PROTOZOA Date Small bacteria/ml.* Lamb No. 93 95 96 Large bacteria/ml, f Lamb No, 93 95 96 OsciUospim/ml.!. Lamb No. 93 95 96 1959 : 2.10 12,10 14-10 1960 : 24,2 16-8 15.10 20,10 27 -10 3.11 9.11 1 WU : 24.1 14-2 1 4〈 ...

Notes: [Auszug] The calves were inoculated by stomach tube at weekly intervals (from 8 days) with a concentrated suspension of rumen ciliates and associated bacteria, and thereafter samples were taken at weekly intervals for visual assessment of bacteria and protozoa and viable counts of three types of organisms. ...

Notes: [Auszug] The method employed was basically to empty the rumen contents from the sheep by way of the rumen canula, to wash out the rumen repeatedly by a method most likely to remove or kill the ciliates and several hours later to return some of the original rumen liquor which had, in the interim, been heated ...

Notes: [Auszug] The effect is markedly similar to that due to the anionic detergent 'TeepoP, as described by Oxford3; but since indole and skatole do not, of course, greatly lower the surface tension of the buffer, another explanation of their action has to be found. Any such explanation must take account of the ...