Publication Date:
2011-11-19
Description:
Many prokaryotic species generate hydrogen sulfide (H(2)S) in their natural environments. However, the biochemistry and physiological role of this gas in nonsulfur bacteria remain largely unknown. Here we demonstrate that inactivation of putative cystathionine beta-synthase, cystathionine gamma-lyase, or 3-mercaptopyruvate sulfurtransferase in Bacillus anthracis, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli suppresses H(2)S production, rendering these pathogens highly sensitive to a multitude of antibiotics. Exogenous H(2)S suppresses this effect. Moreover, in bacteria that normally produce H(2)S and nitric oxide, these two gases act synergistically to sustain growth. The mechanism of gas-mediated antibiotic resistance relies on mitigation of oxidative stress imposed by antibiotics.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Shatalin, Konstantin -- Shatalina, Elena -- Mironov, Alexander -- Nudler, Evgeny -- New York, N.Y. -- Science. 2011 Nov 18;334(6058):986-90. doi: 10.1126/science.1209855.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry, New York University School of Medicine, New York, NY 10016, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22096201" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Anti-Bacterial Agents/*pharmacology
;
Antioxidants/metabolism
;
Bacillus anthracis/drug effects/growth & development/metabolism
;
Bacteria/*drug effects/growth & development/*metabolism
;
Cystathionine beta-Synthase/antagonists & inhibitors/genetics/metabolism
;
Cystathionine gamma-Lyase/antagonists & inhibitors/genetics/metabolism
;
DNA Breaks, Double-Stranded
;
Drug Resistance, Bacterial
;
*Drug Resistance, Multiple, Bacterial
;
Escherichia coli/drug effects/growth & development/metabolism
;
Hydrogen Sulfide/*metabolism/pharmacology
;
Molecular Sequence Data
;
Nitric Oxide/metabolism
;
Oxidative Stress
;
Pseudomonas aeruginosa/drug effects/growth & development/metabolism
;
Staphylococcus aureus/drug effects/growth & development/metabolism
;
Sulfurtransferases/antagonists & inhibitors/genetics/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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