Publication Date:
2015-05-02
Description:
When Staphylococcus aureus undergoes cytokinesis, it builds a septum, generating two hemispherical daughters whose cell walls are only connected via a narrow peripheral ring. We found that resolution of this ring occurred within milliseconds ("popping"), without detectable changes in cell volume. The likelihood of popping depended on cell-wall stress, and the separating cells split open asymmetrically, leaving the daughters connected by a hinge. An elastostatic model of the wall indicated high circumferential stress in the peripheral ring before popping. Last, we observed small perforations in the peripheral ring that are likely initial points of mechanical failure. Thus, the ultrafast daughter cell separation in S. aureus appears to be driven by accumulation of stress in the peripheral ring and exhibits hallmarks of mechanical crack propagation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zhou, Xiaoxue -- Halladin, David K -- Rojas, Enrique R -- Koslover, Elena F -- Lee, Timothy K -- Huang, Kerwyn Casey -- Theriot, Julie A -- 1S10OD01227601/OD/NIH HHS/ -- DP2OD006466/OD/NIH HHS/ -- P50-GM107615/GM/NIGMS NIH HHS/ -- R01 AI036929/AI/NIAID NIH HHS/ -- R01-AI36929/AI/NIAID NIH HHS/ -- R37 AI036929/AI/NIAID NIH HHS/ -- T32 GM007276/GM/NIGMS NIH HHS/ -- T32-GM007276/GM/NIGMS NIH HHS/ -- U54-GM072970/GM/NIGMS NIH HHS/ -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2015 May 1;348(6234):574-8. doi: 10.1126/science.aaa1511.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Chemistry, Stanford University, Stanford, CA 94305, USA. Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA. Howard Hughes Medical Institute (HHMI), Stanford University School of Medicine, Stanford, CA 94305, USA. ; Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA. Howard Hughes Medical Institute (HHMI), Stanford University School of Medicine, Stanford, CA 94305, USA. Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA. ; Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA. Howard Hughes Medical Institute (HHMI), Stanford University School of Medicine, Stanford, CA 94305, USA. Department of Bioengineering, Stanford University, Stanford, CA 94305, USA. ; Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA. Howard Hughes Medical Institute (HHMI), Stanford University School of Medicine, Stanford, CA 94305, USA. ; Department of Bioengineering, Stanford University, Stanford, CA 94305, USA. ; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA. Department of Bioengineering, Stanford University, Stanford, CA 94305, USA. ; Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA. Howard Hughes Medical Institute (HHMI), Stanford University School of Medicine, Stanford, CA 94305, USA. Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA. theriot@stanford.edu.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/25931560" target="_blank"〉PubMed〈/a〉
Keywords:
Cell Wall/physiology/ultrastructure
;
*Cytokinesis
;
Microscopy, Electron, Scanning
;
Microscopy, Video
;
Staphylococcus aureus/cytology/*physiology/ultrastructure
;
Stress, Mechanical
;
Time Factors
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink
