ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    facet.materialart.
    Unknown
    Self-assembling protein microarrays (2004)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2004-07-03
    Description: Protein microarrays provide a powerful tool for the study of protein function. However, they are not widely used, in part because of the challenges in producing proteins to spot on the arrays. We generated protein microarrays by printing complementary DNAs onto glass slides and then translating target proteins with mammalian reticulocyte lysate. Epitope tags fused to the proteins allowed them to be immobilized in situ. This obviated the need to purify proteins, avoided protein stability problems during storage, and captured sufficient protein for functional studies. We used the technology to map pairwise interactions among 29 human DNA replication initiation proteins, recapitulate the regulation of Cdt1 binding to select replication proteins, and map its geminin-binding domain.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ramachandran, Niroshan -- Hainsworth, Eugenie -- Bhullar, Bhupinder -- Eisenstein, Samuel -- Rosen, Benjamin -- Lau, Albert Y -- Walter, Johannes C -- LaBaer, Joshua -- R21 CA99191-01/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 2004 Jul 2;305(5680):86-90.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Harvard Institute of Proteomics, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 320 Charles Street, Cambridge, MA 02141, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15232106" target="_blank"〉PubMed〈/a〉
    Keywords: Cell Cycle Proteins/chemistry/genetics/*metabolism ; Cell-Free System ; *DNA Replication ; DNA, Complementary ; Epitopes ; Geminin ; Humans ; Minichromosome Maintenance Complex Component 2 ; Minichromosome Maintenance Complex Component 6 ; Nuclear Proteins/metabolism ; *Protein Array Analysis/instrumentation/methods ; Protein Binding ; Protein Biosynthesis ; *Protein Interaction Mapping/instrumentation/methods ; Protein Structure, Tertiary ; Proteins/genetics/*metabolism ; Replication Origin ; Transcription, Genetic
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    PAPER CURRENT
  • 2
    facet.materialart.
    Unknown
    Studies on the mineral nutrition of tea (1975)
    Springer
    Details
    Publication Date: 1975-08-01
    Print ISSN: 0032-079X
    Electronic ISSN: 1573-5036
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Springer
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    PAPER CURRENT
  • 3
    Electronic Resource
    Electronic Resource
    Studies on the mineral nutrition of tea (1975)
    Springer
    Plant and soil 43 (1975), S. 259-278 
    Details
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Phosphate fertilizer incorporated in the soil placed around tea plants when planted in the field produced a large improvement in the rate of growth of the plants, thus indicating that tea in acid soils had a demand for phosphate. Yield responses to phosphate fertilizer applied to mature tea were uncommon and the pattern of these confused. This work showed that mature tea will absorb phosphate and give positive yield responses when there is an undisturbed mulch layer on the soil surface. Within this layer phosphate is maintained in a more available form and many highly active roots are formed. These conditions are destroyed by manual weeding; the mulch forms naturally from tea leaves and prunings where no-cultivation herbicidal weed control is practised.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01928491
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Studies on the mineral nutrition of tea (1975)
    Springer
    Plant and soil 43 (1975), S. 259-278 
    Details
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Phosphate fertilizer incorporated in the soil placed around tea plants when planted in the field produced a large improvement in the rate of growth of the plants, thus indicating that tea in acid soils had a demand for phosphate. Yield responses to phosphate fertilizer applied to mature tea were uncommon and the pattern of these confused. This work showed that mature tea will absorb phosphate and give positive yield responses when there is an undisturbed mulch layer on the soil surface. Within this layer phosphate is maintained in a more available form and many highly active roots are formed. These conditions are destroyed by manual weeding; the mulch forms naturally from tea leaves and prunings where no-cultivation herbicidal weed control is practised.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01928491
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...