Publication Date:
2015-07-04
Description:
The global spread of dengue virus (DENV) infections has increased viral genetic diversity, some of which appears associated with greater epidemic potential. The mechanisms governing viral fitness in epidemiological settings, however, remain poorly defined. We identified a determinant of fitness in a foreign dominant (PR-2B) DENV serotype 2 (DENV-2) clade, which emerged during the 1994 epidemic in Puerto Rico and replaced an endemic (PR-1) DENV-2 clade. The PR-2B DENV-2 produced increased levels of subgenomic flavivirus RNA (sfRNA) relative to genomic RNA during replication. PR-2B sfRNA showed sequence-dependent binding to and prevention of tripartite motif 25 (TRIM25) deubiquitylation, which is critical for sustained and amplified retinoic acid-inducible gene 1 (RIG-I)-induced type I interferon expression. Our findings demonstrate a distinctive viral RNA-host protein interaction to evade the innate immune response for increased epidemiological fitness.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Manokaran, Gayathri -- Finol, Esteban -- Wang, Chunling -- Gunaratne, Jayantha -- Bahl, Justin -- Ong, Eugenia Z -- Tan, Hwee Cheng -- Sessions, October M -- Ward, Alex M -- Gubler, Duane J -- Harris, Eva -- Garcia-Blanco, Mariano A -- Ooi, Eng Eong -- R01 AI089526/AI/NIAID NIH HHS/ -- R01 GM087405/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2015 Oct 9;350(6257):217-21. doi: 10.1126/science.aab3369. Epub 2015 Jul 2.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Program in Emerging Infectious Diseases, Duke-National University of Singapore Graduate Medical School, Singapore. Defence Medical and Environmental Research Institute, DSO National Laboratories, Singapore. Yong Loo Lin School of Medicine, National University of Singapore, Singapore. ; Program in Emerging Infectious Diseases, Duke-National University of Singapore Graduate Medical School, Singapore. Yong Loo Lin School of Medicine, National University of Singapore, Singapore. Swiss Tropical and Public Health Institute, Universitat Basel, Switzerland. ; Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, CA, USA. ; Yong Loo Lin School of Medicine, National University of Singapore, Singapore. Institute of Molecular and Cell Biology, Singapore. ; Center for Infectious Diseases, The University of Texas School of Public Health, Houston, TX, USA. ; Program in Emerging Infectious Diseases, Duke-National University of Singapore Graduate Medical School, Singapore. ; Program in Emerging Infectious Diseases, Duke-National University of Singapore Graduate Medical School, Singapore. Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, TX, USA. Department of Molecular Genetics and Microbiology and Center for RNA Biology, Duke University, Durham, NC, USA. ; Program in Emerging Infectious Diseases, Duke-National University of Singapore Graduate Medical School, Singapore. Yong Loo Lin School of Medicine, National University of Singapore, Singapore. Saw Swee Hock School of Public Health, National University of Singapore, Singapore. Singapore-Massachusetts Institute of Technology Alliance in Research and Technology Infectious Disease Interdisciplinary Research Group, Singapore. engeong.ooi@duke-nus.edu.sg.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/26138103" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Biodiversity
;
Cercopithecus aethiops
;
DEAD-box RNA Helicases/metabolism
;
Dengue/epidemiology/*immunology/*virology
;
Dengue Virus/genetics/*physiology
;
Humans
;
*Immunity, Innate
;
Interferon Type I/antagonists & inhibitors/genetics/*immunology
;
Puerto Rico/epidemiology
;
RNA, Viral/genetics/*metabolism
;
Transcription Factors/*metabolism
;
Ubiquitin-Protein Ligases/*metabolism
;
Ubiquitination
;
Vero Cells
;
*Virus Replication
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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