Publication Date:
2003-08-23
Description:
RNA polymerase II (Pol II) transcription through nucleosomes is facilitated in vitro by the protein complex FACT (Facilitates Chromatin Transcription). Here we show that FACT is associated with actively transcribed Pol II genes on Drosophila polytene chromosomes. FACT displays kinetics of recruitment and of chromosome tracking in vivo similar to Pol II and elongation factors Spt5 and Spt6. Interestingly, FACT does not colocalize with Pol III-transcribed genes, which are known to undergo nucleosome transfer rather than disassembly in vitro. Our observations are consistent with FACT being restricted to transcription that involves nucleosome disassembly mechanisms.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Saunders, Abbie -- Werner, Janis -- Andrulis, Erik D -- Nakayama, Takahiro -- Hirose, Susumu -- Reinberg, Danny -- Lis, John T -- GM25232/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2003 Aug 22;301(5636):1094-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14850, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12934007" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cell Nucleolus/metabolism
;
Chromatin/*metabolism
;
*Chromosomal Proteins, Non-Histone
;
DNA-Binding Proteins/genetics
;
Drosophila/*genetics/metabolism
;
Drosophila Proteins/metabolism
;
Fluorescent Antibody Technique
;
Gene Expression Regulation
;
HSP70 Heat-Shock Proteins/genetics
;
Heat-Shock Response
;
*High Mobility Group Proteins
;
Hot Temperature
;
Nucleosomes/metabolism
;
Open Reading Frames
;
Peptide Elongation Factors/metabolism
;
RNA Polymerase II/genetics/*metabolism
;
Transcription Factors/genetics
;
*Transcription, Genetic
;
Transcriptional Elongation Factors/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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