Publication Date:
2006-04-15
Description:
The molecular organization of presynaptic active zones during calcium influx-triggered neurotransmitter release is the focus of intense investigation. The Drosophila coiled-coil domain protein Bruchpilot (BRP) was observed in donut-shaped structures centered at active zones of neuromuscular synapses by using subdiffraction resolution STED (stimulated emission depletion) fluorescence microscopy. At brp mutant active zones, electron-dense projections (T-bars) were entirely lost, Ca2+ channels were reduced in density, evoked vesicle release was depressed, and short-term plasticity was altered. BRP-like proteins seem to establish proximity between Ca2+ channels and vesicles to allow efficient transmitter release and patterned synaptic plasticity.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kittel, Robert J -- Wichmann, Carolin -- Rasse, Tobias M -- Fouquet, Wernher -- Schmidt, Manuela -- Schmid, Andreas -- Wagh, Dhananjay A -- Pawlu, Christian -- Kellner, Robert R -- Willig, Katrin I -- Hell, Stefan W -- Buchner, Erich -- Heckmann, Manfred -- Sigrist, Stephan J -- New York, N.Y. -- Science. 2006 May 19;312(5776):1051-4. Epub 2006 Apr 13.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉European Neuroscience Institute Gottingen, Grisebachstrasse 5, 37077 Gottingen, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16614170" target="_blank"〉PubMed〈/a〉
Keywords:
Action Potentials
;
Animals
;
Calcium Channels/*metabolism
;
Drosophila
;
Drosophila Proteins/genetics/*physiology
;
Female
;
Larva
;
Male
;
Models, Neurological
;
Mutation
;
Nerve Tissue Proteins/metabolism/*physiology
;
Presynaptic Terminals/metabolism
;
Synapses/metabolism/*physiology/ultrastructure
;
Synaptic Vesicles/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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