ISSN:
1573-5044
Keywords:
Agrobacterium tumefaciens
;
genotype effect
;
GUS activity
;
Lamiaceae
;
lavandin
;
shoot regeneration
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Five cultivars of lavandin were compared for their ability to regenerate plantlets in vitro and for their susceptibility to genetic transformation. Both processes were shown to be strongly cultivar-dependent. For regeneration, best results were obtained with cultivar ‘37–70’ which gave an average of 7 shoots from one initial explant after 4 months culture in vitro. The other cultivars produced between 0.5 and 3.5 shoots per explant. These differences were mostly due to the variable efficiency of the shoot elongation and rooting steps. An Agrobacterium-mediated transformation system using the β-glucuronidase and neomycin phosphotransferase II genes was established. The β-glucuronidase expression was analysed for both leaf explants six days after inoculation and kanamycin-resistant calluses obtained after a six-week culture on a selective medium. For each cultivar, kanamycin-resistant calluses showing a β-glucuronidase activity were obtained. The transformation efficiency ranged from 3% for cultivar ‘Certitude’ to 89% for cv. ‘41–70’ and ‘B–110’. Some kanamycin-resistant calluses were organogenic.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1006272520475
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