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  • 1
    ISSN: 1573-515X
    Keywords: binary variables ; fish ; impoundment ; indicator variables ; James Bay ; mercury ; polynomial regression ; Québec ; reservoirs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract Mercury levels in fish in reservoirs and natural lakes have been monitored on a regular basis since 1978 at the La Grande hydroelectric complex located in the James Bay region of Québec, Canada. The main analytical tools historically used were analysis of covariance (ANCOVA), linear regression of the mercury-to-length relationship and Student-Newman-Keuls (SNK) multiple comparisons of mean mercury levels. Inadequacy of linear regression (mercury-to-length relationships are often curvilinear) and difficulties in comparing mean mercury levels when regressions differ lead us to use polynomial regression with indicator variables. For comparisons between years, polynomial regression models relate mercury levels to length (L), length squared (L2), binary (dummy) indicator variables (Bn), each representing a sampled year, and the products of each of these explanatory variables (L × B1, L2 × B1, L × B2, etc.). Optimal transformations of the mercury levels (for normality and homogeneity) were found by the Box-Cox procedure. The models so obtained formed a partially nested series corresponding to four situations: (a) all years are well represented by a single polynomial model; (b) the year-models are of the same shape, but the means may differ; (c) the means are the same, but the year-models differ in shape; (d) both the means and shapes may differ among years. Since year-specific models came from the general one, rigorous statistical comparisons are possible between models. Polynomial regression with indicator variables allows rigorous statistical comparisons of mercury-to-length relationships among years, even when the shape of the relationships differ. It is simple to obtain accurate estimates of mercury levels at standardized length, and multiple comparisons of these estimations are simple to perform. The method can also be applied to spatial analysis (comparison of sampling stations), or to the comparison of different biological forms of the same species (dwarf and normal lake whitefish).
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  • 2
    ISSN: 1573-515X
    Keywords: methylmercury ; mercury bioaccumulation rates ; mercury-to-fish length relationship ; reservoir fish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract Fish mercury concentrations were monitored before and after the construction of the La Grande hydroelectric complex, located in the James Bay region, Québec, Canada. Monitoring results revealed that sympatric populations of dwarf and normal lake whitefish (Coregonus clupeaformis) occur in reservoirs and natural lakes of the area. Dwarf specimens bioaccumulate mercury more rapidly than normal individuals. In natural Sérigny Lake, 6–7 year old dwarf specimens show mean total mercury levels 1.5 times higher than normal individuals of the same age (0.33 vs 0.21 mg kg-1 wet weight). Eleven years after the impoundment of the Caniapiscau reservoir, 6–7 year old dwarf specimens show total mercury concentrations 3 times higher than normal specimens of similar ages (0.74 vs 0.27 mg kg-1). Stomach content analysis showed that although large normal individuals feed on different prey than do dwarf individuals, similar methyl mercury levels were found in prey of both forms. We suggest that dwarf individuals bioaccumulate mercury more rapidly than normal specimens because they begin to mature at an earlier age (usually at age 2 to 3 compared to age 6 for normal specimens) and thus produce proportionally less flesh to dilute the mercury assimilated. Total mercury analysis indicating higher concentrations in flesh than in gonads support this hypothesis. Our results suggest that when monitoring mercury levels in fish in areas where populations of dwarf and normal lake whitefish coexist, dwarf and normal specimens must be distinguished because of their different bioaccumulation rates.
    Type of Medium: Electronic Resource
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