ALBERT — All Library Books, journals and Electronic Records Telegrafenberg

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Genetic characterization, morphometrics and gonad development of induced interspecific hybrids between yellowtail flounder, Pleuronectes ferrugineus (Storer) and winter flounder, Pleuronectes americanus (Walbaum) (2003)

Park, I-S ; Nam, Y K ; Douglas, S E ; [et al.]

Oxford, UK : Blackwell Science Ltd

Aquaculture research 34 (2003), S. 0

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ISSN: 1365-2109

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: Viable interspecific hybrids between yellowtail flounder (Pleuronectes ferrugineus, Storer) and winter flounder (Pleuronectes americanus, Walbaum) were produced by artificial insemination of yellowtail flounder eggs with winter flounder sperm. However, mean fertilization rate, hatching success and early survival up to 3 weeks post hatch were significantly lower than those of parental pure cross controls (P 〈 0.01). Overall, cytogenetic traits (karyological analysis and estimation of cellular DNA contents using flow cytometry) of hybrid flounder were intermediate between the two parental species. Microsatellite assay was used to distinguish the parental genomes in the hybrids; in most cases, one allele was specific to each of the parents. Morphometrics assessed by body proportions indicated that hybrids generally displayed a morphology intermediate between the maternal and paternal species. Interspecific hybrids exhibited abnormal and retarded gonad development in both sexes based on histological analysis of gonads from adult fish. The sterility of the hybrids presents a significant advantage for their use in aquaculture, as potential escapees would not be capable of reproducing in the wild and contaminating natural stocks.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1046/j.1365-2109.2003.00816.x

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2

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Ontogeny of lipase expression in winter flounder (2003)

Murray, H. M. ; Gallant, J. W. ; Perez-Casanova, J. C. ; [et al.]

Oxford, UK : Blackwell Science Ltd

Journal of fish biology 62 (2003), S. 0

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ISSN: 1095-8649

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: The partial sequencing of two lipases from winter flounder Pseudopleuronectes americanus, one most closely related to gastric, lingual and lysosomal acid lipase from other vertebrates and one most closely related to bile salt-activated lipase, is reported. Biochemical analyses of enzymatic activity demonstrated the greater contribution made by bile salt-activated lipase relative to neutral bile salt-independent lipase. Using molecular techniques, the tissue-specific expression of bile salt-activated lipase in pancreatic tissue and acid triacylglycerol lipase in a wide variety of organs was demonstrated. Furthermore, the developmental expression of these types of lipase in larval fish was established.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1046/j.1095-8649.2003.00067.x

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Ontogeny of the stomach in winter flounder: characterization and expression of the pepsinogen and proton pump genes and determination of pepsin activity (1999)

Douglas, S. E. ; Gawlicka, A. ; Mandla, S. ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

Journal of fish biology 55 (1999), S. 0

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ISSN: 1095-8649

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: Clones for two forms of pepsinogen A differing in isoelectric point as well as both α and β subunits of the proton pump were isolated from the stomach of winter flounder Pleuronectes americanus. All clones gave positive hybridization signals with DNA from other flatfish species. Reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that the two forms of pepsinogen were sequentially expressed. Expression of pepsinogen IIa was first detected in 13 days post-hatch (dph) larvae and increased towards the beginning of metamorphosis at 20 dph. Expression of pepsinogen IIb and proton pump genes was first detected at 20 dph and coincided with the appearance of gastric glands and the increase in pepsin activity. The levels of expression of pepsinogen IIb as well as of pepsin activity in 20-dph larvae were similar to those recorded in metamorphosed larvae (27 dph) and juveniles (41 dph). High pepsin activity and levels of expression of the pepsinogen and proton pump genes observed in 20-dph larvae indicate an advanced level of development and functionality of the winter flounder stomach at this stage of ontogeny. This advanced level of the stomach development at 1 week before the currently practised time of weaning suggests that feeding formulated feeds may be initiated earlier.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1095-8649.1999.tb00729.x

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Cellular expression of the pepsinogen and gastric proton pump genes in the stomach of winter flounder as determined by in situ hybridization (2001)

Gawlicka, A. ; Leggiadro, C. T. ; Gallant, J. W. ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

Journal of fish biology 58 (2001), S. 0

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ISSN: 1095-8649

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: The sites of pepsinogen synthesis and hydrochloric acid secretion were localized in the glandular stomach mucosa of winter flounder using in situ hybridization with gastric-specific, biotinylated antisense RNA probes for three genes: pepsinogen IIa, pepsinogen IIb and the a-subunit of the proton pump (H+/K+-ATPase). The spatial expression patterns of the pepsinogen IIa and IIb genes appeared to be the same, but they differed from that of the -proton pump gene. The gastric gland cells contained transcripts (mRNAs) of all three genes, whereas the mucous neck cells and surface mucous cells contained transcript of the a-proton pump only. The expression of the pepsinogen IIa, pepsinogen IIb and α-proton pump gene in the same gastric gland cells indicates that these cells synthesize pepsinogens and secrete hydrochloric acid simultaneously and confirms that they are of the oxynticopeptic type. The presence of the a-proton pump mRNA in the mucous neck and surface mucous cells, shown here for the first time, indicates that these cells are capable of secreting hydrochloric acid. The physiological importance of hydrochloric acid secretion by these two cell types remains unknown.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1095-8649.2001.tb02271.x

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5

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Molecular investigation of aminopeptidase N expression in the winter flounder, Pleuronectes americanus (1999)

Douglas, S. E. ; Gallant, J. W. ; Bullerwell, C. E.

Oxford, UK : Blackwell Science, Ltd

Journal of applied ichthyology 15 (1999), S. 0

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ISSN: 1439-0426

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: The primary structure of a polypeptide with significant similarity to human aminopeptidase N (EC 3.4.11.2) was deduced from the sequence of two overlapping cDNA clones derived from winter flounder (Pleuronectes americanus, Walbaum) intestinal RNA and a partial genomic clone derived from winter flounder DNA. The deduced amino acid sequence (975 amino acids) is comprised of an N-terminal cytosolic portion of seven amino acid residues, followed by a 24-residue transmembrane anchor region, a 38-residue serine/threonine-rich junction and a 906-residue enzymatic domain that protrudes from the apical membrane of the enterocyte. A highly conserved signature sequence characteristic of zinc-dependent metallopeptidases (HExxH) is present, as well as seven putative glycosylation sites (NxS/T). Fourteen introns are present in the 7.53 kb portion of the gene that was cloned and sequenced. Reverse transcriptase polymerase chain reaction assays using primers spanning intron/exon boundaries were used to determine the timing of expression of this gene in larval winter flounder. This represents the first aminopeptidase N sequence to be determined from a teleost fish and underscores the utility of molecular biological information in the investigation of larval fish digestion.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1046/j.1439-0426.1999.00117.x

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