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  • 1
    Electronic Resource
    Electronic Resource
    Expression of the GUS-gene in the monocot tulip after introduction by particle bombardment and Agrobacterium (1992)
    Springer
    Plant cell reports 11 (1992), S. 76-80 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Gene transfer to the monocotyledon tulip (Tulipa sp. L.) was obtained both by particle bombardment and Agrobacterium transformation. Using a Particle Delivery System, transient expression of the reporter gene for ßglucuronidase was demonstrated. It was shown that the CAMV 35S as well as the TR2' promoter were active in flower stem expiants. Various wildtype and disarmed Agrobacterium strains, harbouring the 35S GUSintron gene on a binary plasmid, were used for infection of flower stem expiants of 7 cultivars and 7 botanical Tulipa species. In nine genotypes the GUSintron gene was expressed, despite the fact that tulip tissue did not produce detectable amounts of virulence-inducing substances. Agrobacterium rhizogenes appeared to be most effective in gene transfer to tulip tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00235257
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  • 2
    Electronic Resource
    Electronic Resource
    Activity of constitutive promoters in various species from the Liliaceae (1995)
    Springer
    Plant molecular biology 28 (1995), S. 949-955 
    Details
    ISSN: 1573-5028
    Keywords: Liliaceae ; tulip ; lily ; leek ; monocot intron ; particle bombardment ; promoter activity ; transient expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In this paper we first review literature on the performance of various promoters in monocotyledonous species. In general, promoters isolated from monocots show a higher activity in monocot species. Moreover, the presence of an intron between the promoter and reporter gene increases transcription levels. We used the same approach to study gene expression in Liliaceae. The activities of the CaMV 35S, maize Adh1-based pEmu, rice Act1 and maize Ubi promoters, coupled to the β-glucuronidase (gus) reporter gene, were evaluated for transient gene expression upon particle bombardment of tissues of tobacco, rice, tulip, lily and leek. Although monocot promoters performed very well in rice tissues, the results of this study show that this cannot be generalized for other monocot species. The transcription inducing effects of monocot promoters were less pronounced or even absent in tissues of Liliaceae, while the presence of an intron between promoter and gus gene reduced promoter activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00042079
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of Alternaria alternata f.sp. lycopersici toxins on pollen (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 204-208 
    Details
    ISSN: 1432-2242
    Keywords: Alternaria alternata f.sp. lycopersici ; Pollen ; Gametophytic-sporophytic gene expression ; Pollen selection ; Host-specific toxins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Effects of the phytotoxic compounds (AAL-toxins) isolated from cell-free culture filtrates of Alternaria alternata f.sp. lycopersici on in vitro pollen development were studied. AAL-toxins inhibited both germination and tube growth of pollen from several Lycopersicon genotypes. Pollen from susceptible genotypes, however, was more sensitive for AAL-toxins than pollen from resistant plants, while pollen of species not belonging to the host range of the fungus was not significantly affected by the tested toxin concentrations. AAL-toxins elicit symptoms in detached leaf bioassays indistinguishable from those observed on leaves of fungal infected tomato plants, and toxins play a major role in the pathogenesis. Apparently, pathogenesis-related processes and mechanisms involved in disease resistance are expressed in both vegetative and generative tissues. This overlap in gene expression between the sporophytic and gametophytic level of a plant may be advantageously utilized in plant breeding programmes. Pollen may be used to distinguish susceptible and resistant plants and to select for resistances and tolerances against phytotoxins and other selective agents.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00257847
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  • 4
    Electronic Resource
    Electronic Resource
    Effect of phytotoxic compounds produced by Clavibacter michiganensis subsp. michiganensis on resistant and susceptible tomato plants (1989)
    Springer
    European journal of plant pathology 95 (1989), S. 107-117 
    Details
    ISSN: 1573-8469
    Keywords: bacterial canker ; Lycopersicon esculentum ; Lycopersicon peruvianum ; virulence ; bioassay ; non-specific toxin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Samenvatting Een fytotoxische fractie werd geïsoleerd uit cultuurfiltraat vanClavibacter michiganensis subsp.michiganensis, de veroorzaker van de bacterieverwelkingsziekte bij tomaat. Een eerste karakterisering toonde aan dat deze toxische fractie hoog-moleculaire component(en) bevat, bestaande uit polysacchariden en een gering percentage eiwit. Dit is in overeenstemming met toxines vanC. michiganensis subsp.michiganensis die al eerder beschreven zijn. Deze hoogmoleculaire toxische fractie was in staat verwelking te induceren van stengeltoppen van verschillendeLycopersicon esculentum enL. peruvianum genotypen in een bioassay. Gewichtsverandering van de stengeltoppen, uitgedrukt als percentage ten opzichte van het begingewicht, werd gebruikt als parameter voor verwelking. De toxische fractie reageerde niet-specifiek in de bioassay, want er werd geen verschil gevonden in respons van (partieel) resistente en gevoelige genotypen. Er bleek geen correlatie te zijn tussen de mate van virulentie van verschillende isolaten vanC. michiganensis subsp.michiganensis en de hoeveelheid van de toxische fractie geproduceerd in vitro. Het mogelijke gebruik van deze hoogmoleculaire toxische fractie als selectief agens bij in vitro selectie zal nader onderzocht worden.
    Notes: Abstract A phytotoxic fraction of high molecular weight was isolated from the culture filtrate ofClavibacter michiganensis subsp.michiganensis, the causal agent of bacterial canker of tomato, and partly purified. This high molecular weight fraction consists of sugars and a minor protein moiety and is therefore probably of similar nature to that of the toxin fromC. michiganensis subsp.michiganensis reported earlier in literature. The high molecular weight fraction was albe to induce wilting, the predominant symptom of the disease, as shown in a bioassay with tomato cuttings. However, this wilting reaction turned out to be non-specific in the bioassay, since (partially) resistant and susceptible genotypes responded similarly. No correlation could be found between the degree of virulence of fiveC. michiganensis subsp.michiganensis strains and the amount of the phytotoxic high molecular weight fraction produced in vitro. As the isolated high molecular weight fraction showed a phytotoxic effect on tomato plants it is worthwhile to test its potential for use as a selective agent in in vitro selection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01997478
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  • 5
    Electronic Resource
    Electronic Resource
    Improvement of adventitious shoot formation from carnation leaf explants (1994)
    Springer
    Plant cell, tissue and organ culture 37 (1994), S. 87-90 
    Details
    ISSN: 1573-5044
    Keywords: Dianthus caryophyllus ; shoot formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Adventitious shoot formation from leaf explants of carnation (Dianthus caryophyllus L.) was investigated. The two leaves from one node of in vitro-grown plants showed different shoot-forming potential, depending on the order in which the leaves were removed from the stem. The leaf removed second formed more shoots and also had a large amount of adhering stem tissue. Explants with equal amounts of adhering stem tissue were obtained by making two incisions through the fused leaf bases, prior to their removal, resulting in an improved shoot formation. The procedure developed for leaf explants from in vitro-grown plants was also applied to leaf explants from greenhousegrown plants. Shoot formation from leaf explants taken from greenhouse-grown plants was further improved by cutting the leaf explant longitudinally into two parts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00048123
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  • 6
    Electronic Resource
    Electronic Resource
    Plant regeneration from cucumber protoplasts (1988)
    Springer
    Plant cell, tissue and organ culture 12 (1988), S. 147-150 
    Details
    ISSN: 1573-5044
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040077
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  • 7
    Electronic Resource
    Electronic Resource
    High frequency somatic embryogenesis and plant regeneration from zygotic embryo-derived callus cultures of three Allium species (1992)
    Springer
    Plant cell, tissue and organ culture 30 (1992), S. 181-191 
    Details
    ISSN: 1573-5044
    Keywords: abscisic acid ; Allium cepa ; Allium fistulosum ; Allium porrum ; MS medium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The plant regeneration ability of zygotic embryo-derived callus cultures was studied for 12 A. cepa varieties and accessions, two A. fistulosum varieties, one A. fistulosum x A. cepa interspecific hybrid and two A. porrum varieties. Compact embryogenic callus was induced on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid. The embryogenic calluses of all three Allium species were similar in appearance. For all accessions tested plants could be regenerated at a high frequency from this compact callus through somatic embryogenesis, when using kinetin supplemented MS medium (regeneration medium). Addition of abscisic acid to the regeneration medium stimulated the formation of both somatic embryos and shoots for a number of varieties. Concerning shoot regeneration from callus cultures, significant differences existed between genotypes of all accessions except one.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040020
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