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  • 1
    Electronic Resource
    Electronic Resource
    Expression of chimaeric genes transferred into plant cells using a Ti-plasmid-derived vector (1983)
    [s.l.] : Nature Publishing Group
    Nature 303 (1983), S. 209-213 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Foreign genes introduced into plant cells with Ti-plasmid vectors are not expressed. We have constructed an expression vector derived from the promoter sequence of nopaline synthase, and have inserted the coding sequences of the octopine synthase gene and a chloramphenicol acetyltransferase gene ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/303209a0
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  • 2
    Electronic Resource
    Electronic Resource
    Boosting heterologous protein production in transgenic dicotyledonous seeds using Phaseolus vulgaris regulatory sequences (2002)
    [s.l.] : Nature Publishing Group
    Nature biotechnology 20 (2002), S. 1265-1268 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Over the past decade, several high value proteins have been produced in different transgenic plant tissues such as leaves, tubers, and seeds. Despite recent advances, many heterologous proteins accumulate to low concentrations, and the optimization of expression cassettes to make in planta ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt755
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  • 3
    Electronic Resource
    Electronic Resource
    The Agrobacterium tumefaciens Ti plasmid as a host vector system for introducing foreign DNA in plant cells (1980)
    [s.l.] : Nature Publishing Group
    Nature 287 (1980), S. 654-656 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Tn7, which encodes streptomycin, spectinomycin and tri-methoprim resistance, was introduced into the chromosome of strain T37 Noccl. An RP4 ::Tn7 plasmid9, pGVl (our isolate) was transferred from the auxotrophic Escherichia coli strain GV1000 (rf. 11) to strain T37 Noccl by plate conjugation12. A ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/287654a0
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  • 4
    Electronic Resource
    Electronic Resource
    Homologous DNA sequences in different Ti-plasmids are essential for oncogenicity (1978)
    [s.l.] : Nature Publishing Group
    Nature 275 (1978), S. 150-153 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The Ti-plasmid of strain ACH5 was used as an example of an octopine Ti-plasmid. This strain contains only one large plasmid (molecular weight 120 106), which was shown by heteroduplex analysis8 to be completely homologous to other octopine Ti-plasmids, such as the Ti-plasmid of strain B6S3. ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/275150a0
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  • 5
    Electronic Resource
    Electronic Resource
    Expression of chimaeric genes transferred into plant cells using a Ti-plasmid-derived vector (1983)
    [s.l.] : Nature Publishing Group
    Nature 303 (1983), S. 209-213 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Foreign genes introduced into plant cells with Ti-plasmid vectors are not expressed. We have constructed an expression vector derived from the promoter sequence of nopaline synthase, and have inserted the coding sequences of the octopine synthase gene and a chloramphenicol acetyltransferase gene ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/303209a0
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  • 6
    Electronic Resource
    Electronic Resource
    Effect of T-DNA configuration on transgene expression (1992)
    Springer
    Molecular genetics and genomics 235 (1992), S. 389-396 
    Details
    ISSN: 1617-4623
    Keywords: Agrobacterium tumefaciens ; Inter-transformant variability ; Nicotiana tabacum ; Position effect ; Transcription interference
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary T-DNA vectors were constructed which carry a β-glucuronidase (gusA) gene fused to the promoter of the nopaline synthase (nos) gene and the 3′ end of the octopine synthase (ocs) gene. This reporter gene was cloned at different locations and orientations towards the right T-DNA border. For each construct, between 30 and 60 stably transformed calli were analysed for β-glucuronidase activity. Depending on the T-DNA configuration, distinct populations of gusA-expressing calli were obtained. Placing the reporter gene in the middle of the T-DNA results in relatively low expression levels and a limited inter-transformant variability. Placing the gene with its promoter next to the right border led to an increase in both the mean activity and the variability level. With this construct, some of the calli expressed the gusA gene at levels four to five times higher than the mean. In all these series, at least 30% of the calli contained reporter gene activities that were less than half of the mean expression level. Separating the gusA gene from the right T-DNA border by an additional 3′-untranslated region, derived from the nos gene, resulted in an increase in the mean expression to a level almost four times higher than that of constructions carrying the reporter gene in the middle of the T-DNA. Moreover, the number of transformants with extremely low activities decreased by at least 50% and this resulted in significantly lower inter-transformant variability independently of the orientation of the reporter gene on the T-DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00279385
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  • 7
    Electronic Resource
    Electronic Resource
    The plantibody approach: expression of antibody genes in plants to modulate plant metabolism or to obtain pathogen resistance (2000)
    Springer
    Plant molecular biology 43 (2000), S. 419-428 
    Details
    ISSN: 1573-5028
    Keywords: antibody production ; gene inactivation ; immunomodulation ; phage display ; plantibody ; reducing environment ; single-chain variable fragment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Immunomodulation is a molecular technique that allows the interference with cellular metabolism or pathogen infectivity by the ectopic expression of genes encoding antibodies or antibody fragments. In recent years, several reports have proven the value of this tool in plant research for modulation of phytohormone activity and for blocking plant-pathogen infection. Efficient application of the plantibody approach requires different levels of investigation. First of all, methods have to be available to clone efficiently the genes coding for antibodies or antibody fragments that bind the target antigen. Secondly, conditions to obtain high accumulation of antigen-binding antibodies and antibody fragments in plants are being investigated and optimized. Thirdly, different strategies are being evaluated to interfere with the function of the target molecule, thus enabling immunomodulation of metabolism or pathogen infectivity. In the near future, optimized antibody gene isolation and expression, especially in reducing subcellular environments, such as the cytosol and nucleus, should turn immunomodulation into a powerful and attractive tool for gene inactivation, complementary to the classical antisense and co-suppression approaches.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006471528756
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  • 8
    Electronic Resource
    Electronic Resource
    Different 5′ leader sequences modulate β-glucuronidase accumulation levels in transgenic Nicotiana tabacum plants (1955)
    Springer
    Euphytica 85 (1955), S. 209-216 
    Details
    ISSN: 1573-5060
    Keywords: β-glucuronidase ; plant ; silencing ; translational control ; 5′-untranslated region ; variation of gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three random synthetic leaders and three naturally-occurring leaders, the tobacco mosaic virus (TMV) coat protein, the satellite tobacco necrosis virus (STNV) and the plant chlorophyll a/b-binding protein (Cab22L), were shown to modulate the β-glucuronidase reporter protein accumulation levels in transient expression experiments. The same chimeric constructs also confer differential distribution patterns of reporter protein accumulation in stably-transformed tobacco calli or regenerated transgenic plants. When the highest expression levels with a given leader are compared, the 31-nucleotide random leader stimulates translation 20- and 100-fold relative to the 9- and 4- nucleotide synthetic leaders respectively. However, this 31-nucleotide random leader is approx. 2 to 3-fold weaker than the 30-nucleotide STNV leader and even 5-fold weaker than both the 79-nucleotide TMV leader and the 66-nucleotide Cab22L leader. These results confirm the findings in transient expression experiments and stress the importance of the 5′-untranslated region for the production of heterologous proteins in transgenic plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023950
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  • 9
    Electronic Resource
    Electronic Resource
    Frequencies of simultaneous transformation with different T-DNAs and their relevance to the Agrobacterium/plant cell interaction (1985)
    Springer
    Molecular genetics and genomics 201 (1985), S. 477-484 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We investigated whether the efficiency of transformation of plant cells by Agrobacterium tumefaciens during cocultivation is limited by the properties of the plant cells or by the infecting bacteria. Therefore, tobacco protoplasts were infected by cocultivation with two different agrobacteria strains carrying Ti plasmids with distinguishable T-DNAs. These T-DNAs cotransform plant cells at a frequency equal to the product of their independent transformation frequencies, which indicates that all plant cells are equally competent. On the other hand, when these T-DNAs are located on the same Ti plasmid vector within one bacterial strain, the cotransformation frequency is significantly higher than the product of the single transformation frequencies. We interpret these results to indicate that transformation is limited more by the establishment of effective bacteria/plant cell interaction than by (i) the process of DNA integration and (ii) by the number of plant cells capable of being transformed by Agrobacterium. We found that most plant cells are transformed by only one or a few agrobacteria. Analysis of the number of T-DNA copies in these clonally transformed lines indicates amplification of the original, infecting T-region copy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331342
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  • 10
    Electronic Resource
    Electronic Resource
    Assembly of an antibody and its derived antibody fragment inNicotiana andArabidopsis (1993)
    Springer
    Transgenic research 2 (1993), S. 227-237 
    Details
    ISSN: 1573-9368
    Keywords: CaMV 35S promoter ; Fab fragment ; inter-transformant variability ; monoclonal antibody ; self-assembly ; transgenic plants ; Arabidopsis thaliana ; Nicotiana tabacum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The yield and assembly of an IgG1 antibody and its derived Fab fragment were compared inNicotiana andArabidopsis. The results obtained showed a lot of interclonal variability. For 45% of the primary transgenic calluses, antigen-binding entities represented less than 0.1% of the total soluble protein (TSP). Only two of the 103 analysed transformants contained more than 1% of antigen-binding protein, with 1.26% being the highest yield. Analogous amounts of complete antibody and Fab accumulated in primary callus tissue. Moreover, yields were in the same range for both species as far as primary callus tissue is concerned. However, the accumulation of the Fab fragment in leaf tissue of regenerated plants differed significantly betweenNicotiana andArabidopsis. The Fab fragment accumulated to only 0.044% of TSP inNicotiana leaves but up to 1.3% inArabidopsis leaves. Furthermore, both species showed differences in the assembly pattern of the complete antibody. WhereasArabidopsis contained primarily fully assembled antibodies of 150 kDa,Nicotiana showed an abundance of fragments in the 50 kDa range.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01977353
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