Publication Date:
2019
Description:
Abstract
Cervical squamous cell carcinoma (CSCC) accounts for a significant proportion of cervical cancer, and thus there is a need for novel and noninvasive diagnostic biomarkers for this malignancy. In the current study, we performed integrated analysis of a datset from the Gene Expression Omnibus to identify differentially expressed genes (DEGs) and differentially expressed miRNAs (DEmiRNAs) between CSCC, cervical intraepithelial neoplasia (CIN) and healthy controls. We further established protein‐protein interaction (PPI) and DEmiRNA‐target gene interaction networks, and performed functional annotation of the target genes of DEmiRNAs. In total, we identified 1375 DEGs and 19 DEmiRNAs in CIN vs. N, and 2235 DEGs and 33 DEmiRNAs in CSCC vs. CIN by integrated analysis. Our PPI network indicates that the common DEGs CDK1, CCND1, ESR1 and AURKA are the top four hub genes. P53 and prostate cancer were identified as significantly enriched signaling pathways of common DEGs and DEmiRNA targets, respectively. We validated that expression levels of three DEGs (TYMS, SASH1 and CDK1) and one DEmiRNA of hsa‐miR‐99a were altered in blood samples of patients with CSCC. In conclusion, a total of four DEGs (TYMS, SASH1, CDK1 and AURKA) and two DEmiRNAs (hsa‐miR‐21 and hsa‐miR‐99a) may be involved in the pathogenesis of CIN and the progression of CIN into CSCC. Of these, TYMS is predicted to be regulated by hsa‐miR‐99a and SASH1 to be regulated by hsa‐miR‐21.
Electronic ISSN:
2211-5463
Topics:
Biology
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