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  • 1
    Electronic Resource
    Electronic Resource
    Identification of a phosphorylation site and functional analysis of conserved aspartic acid residues of OmpR, a transcriptional activator for ompF and ompC in Escherichia coli (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 10 (1993), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: In Escherichia coli the OmpR and EnvZ proteins regulate the expression of the outer membrane porin proteins OmpC and OmpF. EnvZ and OmpR belong to a family of sensor/effector protein pairs that control adaptation to a variety of environmental conditions. EnvZ acts as the sensor protein that phosphorylates OmpR, which in turn regulates porin gene expression. The level of phosphorylated OmpR appears to be a determining factor for ompC and ompF regulation. Phosphorylation of OmpR is considered to occur at one or more aspartic acid residues (Asp-11, Asp-12 and/or Asp-55) that are highly conserved among the effector proteins. In this report we biochemically characterized the aspartic acid residue(s) in OmpR that were phosphorylated by EnvZ. Reduction of aspartyl phosphate residues in the amino-terminal domain of OmpR with [3H]-NaBH4 indicated that Asp-55 was a primary site of modification. We further studied the role of the highly conserved aspartate residues by creating OmpR mutants having aspartate to alanine substitutions at positions 11 (D11A), 12 (D12A) and 55 (D55A). Studies of ompF and ompC expression as well as in vivo and in vitro phosphorylation experiments also demonstrated that while Asp-55 is the primary phosphate acceptor site in OmpR, Asp-11 may also serve as a phosphorylation site, particularly in the absence of Asp-55.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1993.tb00974.x
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  • 2
    Electronic Resource
    Electronic Resource
    Lagrangian flows: The dynamics of globally minimizing orbits-II (1997)
    Springer
    Bulletin of the Brazilian Mathematical Society 28 (1997), S. 155-196 
    Details
    ISSN: 1678-7714
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract Define the critical levelc(L) of a convex superlinear LagragianL as the infimum of thek ∈ ℝsuch that the LagragianL+k has minimizers with fixed endpoints and free time interval. We provide proofs for Mañé's statements [7] characterizingc(L) in termos of minimizing measures ofL, and also giving graph, recurrence covering and cohomology properties for minimizers ofL+c(L). It is also proven thatc(L) is the infimum of the energy levelsk such that the following for of Tonelli's theorem holds:There exists minimizers of the L+k-action joining any two points in the projection of E=k among curves with energy k.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01233390
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  • 3
    Electronic Resource
    Electronic Resource
    Modulation of GT-1 DNA-binding activity by calcium-dependent phosphorylation (1999)
    Springer
    Plant molecular biology 40 (1999), S. 373-386 
    Details
    ISSN: 1573-5028
    Keywords: calcium/calmodulin kinase II ; calcium-dependent protein kinase ; GT-1 ; rbcS-3A ; phytochrome signal transduction ; transcription factor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The analysis of pea rbcS-3A promoter sequence showed that BoxII was necessary for the control of rbcS-3A gene expression by light. GT-1, a DNA-binding protein that interacts with BoxII in vitro, is a good candidate for being a light-modulated molecular switch controlling gene expression. However, the relationship between GT-1 activity and light-responsive gene activation still remains hypothetical. Because no marked de novo synthesis was detected after light treatment, light may induce post-translational modifications of GT-1 such as phosphorylation or dephosphorylation. Here, we show that recombinant GT-1 (hGT-1) of Arabidopsis can be phosphorylated by various mammalian kinase activities in vitro. Whereas phosphorylation by casein kinase II had no apparent effect on hGT-1 DNA binding, phosphorylation by calcium/calmodulin kinase II (CaMKII) increased the binding activity 10–20-fold. Mass spectrometry analyses of the phosphorylated hGT-1 showed that amongst the 6 potential phosphorylatable residues (T86, T133, S175, T179, S198 and T278), only T133 and S198 are heavily modified. Analyses of mutants altered at T86, T133, S175, T179, S198 and T278 demonstrated that phosphorylation of T133 can account for most of the stimulation of DNA-binding activity by CaMKII, indicating that this residue plays an important role in hGT-1/BoxII interaction. We further showed that nuclear GT-1 DNA-binding activity to BoxII was reduced by treatment with calf intestine phosphatase in extracts prepared from light-grown plants but not from etiolated plants. Taken together, our results suggest that GT-1 may act as a molecular switch modulated by calcium-dependent phosphorylation and dephosphorylation in response to light signals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006131330930
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  • 4
    Electronic Resource
    Electronic Resource
    Simulation of Nitrate-Nitrogen Dynamics for Cropping Systems with Different Rooting Depths (2000)
    Springer
    Soil Science Society of America journal 64 (2000), S. 1050-1054 
    Details
    ISSN: 1435-0661
    Keywords: BMPs, best management practices BRD, bottom of the root depth NLEAP, Nitrate Leaching and Economic Analysis Package RSN, residual soil NO−3 SLVWQDP, San Luis Valley Water Quality Demonstration Project
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: − 3–N (RSN) for the root zone of each crop grown in the region and for a similar soil depth for these systems (e.g., 0–0.91 m). The improved NLEAP version 1.20 simulates maximum rooting depth to the nearest 0.03 m and RSN in multiple soil depths. These new features allowed us to simulate the effect of BMPs on RSN for the root zones of shallower-rooted crops such as lettuce (Lactuca sativa L.) (0–0.37 m), potato (Solanum tuberosum L.) (0–0.40 m), and deeper-rooted crops such as small grains (0–0.61 to 0–0.84 m). NLEAP simulated RSN for a soil depth identical for all of these cropping systems (0–0.91 m) (P 〈 0.001). This new version can be used by extension agents, farmers, consultants, and others to evaluate the effect of BMPs on soil NO− 3–N dynamics for different rooting zones and for similar soil depths in the agricultural system, a capability that was not available with the previous version of NLEAP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/
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  • 5
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    Estructura comunitaria del macrozoobentos de sustrato arenoso de la caleta puerto inglés, Bahía de Ilo-Moquegua, Perú (2020)
    Details
    Publication Date: 2021-05-19
    Description: Durante el verano de 2017, en la Caleta de Puerto Inglés de la ciudad de Ilo-región Moquegua-Perú, se determinó la estructura comunitaria del macrozoobentos. Para los muestreos se utilizaron transectos perpendiculares a la línea costera en el nivel mesolitoral e infralitoral de sustrato arenoso. Se empleó la metodología del cilindro Penchaszadeh y se determinó la riqueza específica, abundancia y distribución verti- cal del macrozoobentos. Al analizar la composición taxonómica de la macrofauna destacó la presencia de tres phyla principales: Mollusca (15 especies), Arthropoda (12 especies) y Annelida (5 especies). Sin embargo, la abundancia por phyla fue inversa, siendo Annelida (69%), Arthropoda (14%) y Mollusca (13.5%) los grupos que tuvieron mayor abundancia. Se determinó la diversidad a cada estrato de muestreo a través de los índices de Shannon – Weaver (H'), Equidad de Pielou (J') y Simpson (λ). Además, se comparó la estructura comunitaria de cada estrato de muestreo mediante los índices de Similitud de BrayCurtis, análisis de escalamiento multidimensional no métrico (nMDS) y curvas de K-dominancia
    Description: The community structure of the macrozoobenthos was determined during the summer of 2017 in English Port Caleta of the city of Ilo- Moquegua-Peru region. For the sampling, perpendicular transects to the shoreline were used at mesolitoral and infralitoral levels of sandy substrate. The Penchaszadeh cylinder methodology was used, and the specific wealth, abundance and vertical distribution of macrozooben- thos was determined. When analyzing the taxonomic composition of the macrofauna, it highlighted the presence of three main phyla: Mollusca (15 species), Arthropoda (12 species) and Annelida (5 species). However, the abundance by phyla was inverse, being Annelida (69%), Arthropoda (14%) and Mollusca (13.5%) the groups that had greater abundance. Diversi- ty was determined at each sampling stratum through the Shannon - Weaver (H '), Pielou Equity (J') and Simpson (λ) indexes. Further more, the community structure was compared to each sampling stratum using the Similarity of Bray Curtis indexes, non-metric multi- dimensional scaling analysis (nMDS) and K dominance curves lusca (15 species), Arthropoda (12 species) and Annelida (5 species). However, the abundance by phyla was inverse, being Annelida (69%), Arthropoda (14%) and Mollusca (13.5%) the groups that had greater abundance. Diversi- ty was determined at each sampling stratum through the Shannon - Weaver (H '), Pielou Equity (J') and Simpson (λ) indexes. Further- more, the community structure was compared to each sampling stratum using the Similar- ity of Bray Curtis indexes, non-metric multi- dimensional scaling analysis (nMDS) and K dominance curves.
    Description: Published
    Keywords: Sustrato arenoso ; Macrozoobentos ; Estructura comunitaria ; Ilo ; Perú ; Sandy substrate ; Macrozoobenthos ; Community structure ; Ilo ; Peru
    Repository Name: AquaDocs
    Type: Journal Contribution , Not Known
    Format: pp.49-71
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  • 6
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    Conservación del joturo (Nandopsis ramsdeni), pez fluvial endémico de Guantánamo y amenazado de extinción. (2019)
    Details
    Publication Date: 2021-05-19
    Description: Con el presente trabajo se continuó el estudio y la evaluación de sitios y sistemas fluviales con presencia del joturo o biajaca del Guaso (Nandopsis ramsdeni Fowler, 1938), pez de agua dulce endémico del sistema fluvial de Guantánamo, también encontrado en algunos ríos adyacentes, en las provincias de Santiago de Cuba y Holguín. Se demostró que la población de Arroyo Hondo una de las localidades tipo de este pez se extinguió, se pudo comprobar que la presencia de especies introducidas e invasoras de la ictiofauna fluvial en algunos ríos ha diezmado a la biajaca del Guaso, por ejemplo, el pez gato (Clarias gariepinus) y se identifica al Parque Nacional Alejandro de Humboldt como el sitio más importante de su confinamiento poblacional. El joturo o biajaca del Guaso mostró una elevada afinidad por los fondos rocosos unido a su condición de ingeniera de hábitat le garantiza protección efectiva, ya que en más del 52 % de estos ríos predominan los fondos rocosos. Con los métodos cartográficos y el Sistema de Información Geográfica se diseñó un mapa del área, se actualizó el inventario ictiológico con determinación de presencia/ausencia del taxón, se hizo un análisis de morfometría comparada y se aplicaron entrevistas a pescadores de subsistencia en las comunidades locales.
    Description: With this work continued the study and evaluation of sites and river systems with the presence of the joturo or biajaca del Guaso (Nandopsis ramsdeni Fowler, 1938), freshwater fish endemic to the Guantánamo fluvial system, also found in some adjacent rivers, in the provinces of Santiago de Cuba and Holguin. It was shown that the population of Arroyo Hondo, one of the typical localities of this fish, became extinct. It was found that the presence of introduced and invasive species of the fluvial ichthyofauna in some rivers has decimated the joturo, for example, the fish cat (Clarias gariepinus) and Alejandro de Humboldt National Park is identified as the most important site of its population confinement. The joturo or biajaca of Guaso showed a high affinity for the rocky bottoms together with its condition of habitat engineer guarantees effective protection, since in more than 52 % of these rivers the rocky bottoms predominate. With the cartographic methods and the Geographical Information System, a map of the area was designed, the fish inventory was updated with determination of the presence/absence of the taxon, a comparative morphometry analysis was made and interviews were applied to subsistence fishermen in the local communities.
    Description: Published
    Keywords: Joturo ; Pérdida de hábitat ; Confinamiento y conservación ; Loss of habitat ; Confinement and conservation
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.46-54
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  • 7
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    Lista sistemática de isópodos marinos del Perú, primer registro de Isocladus bahamondei Carvacho, 1997 (CRUSTACEA, PERACARIDA) y aportes de su ecología para la costa peruana (2022)
    Details
    Publication Date: 2022-01-29
    Description: Existe poco conocimiento sobre los estudios taxonómicos en los ecosistemas marinos del Perú, entre ellos los isópodos marinos son un grupo poco estudiado. El objetivo de esta investigación es actualizar el listado de isópodos marinos del Perú y presentar el nuevo registro de Isocladus bahamondei Carvacho, 1977. Se estudiaron tres playas: Tres Hermanas, Malecón Costeo y Puerto Inglés. La recolección se realizó de forma manual en la zona mesolitoral con la utilización de un marco cuadrado de 25 x 25 cm. La playa que tuvo mayores valores de abundancia y biomasa de I. bahamondei fue Puerto Inglés (179 ind/ m2 y 1.45 g/m2). El mayor pico de esos valores se encontró entre los meses de agosto y septiembre (final de invierno y principios de primavera). En este trabajo se actualiza el listado sistemático de isópodos peruanos y se amplía el rango de distribución de I. bahamondei hacia el norte del ecosistema de Humboldt.
    Description: There is little knowledge about taxonomic studies in the marine ecosystems of Peru, among them the marine isopods are a little studied group. The objective of this study is to update the list of marine isopods of Peru and present the new record of Isocladus bahamondei Carvacho, 1977. Three beaches were studied: Tres Hermanas, Malecón Costero and Puerto Inglés. The collection was carried out manually in the mesolittoral zone using a square frame of 25 x 25 cm. The beach with the highest abundance and biomass of I. bahamondei was Puerto Inglés (179 ind/m2 and 1.45 g/m2). The highest abundance and density peaks were found between August and September (late winterand early spring). In this work the systematic list of Peruvian isopods is updated and the distribution range of I. bahamondei is expanded towards the north of the Humboldt ecosystem.
    Description: Published
    Description: Refereed
    Keywords: abundancia ; biomasa ; mesolitoral rocoso ; Sphaeromatidae ; taxonomía ; abundance ; biomass ; rocky intertidal zone ; taxonomy
    Repository Name: AquaDocs
    Type: Journal Contribution
    Format: pp.22-35
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  • 8
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    Interactions of Stover and Nitrogen Management on Soil Microbial Community and Labile Carbon under Irrigated No-Till Corn (2018)
    Wiley
    Details
    Publication Date: 2018-02-08
    Print ISSN: 0361-5995
    Electronic ISSN: 1435-0661
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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  • 9
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    Simulation of Nitrate-Nitrogen Dynamics for Cropping Systems with Different Rooting Depths (2000)
    Wiley
    Details
    Publication Date: 2000-05-01
    Print ISSN: 0361-5995
    Electronic ISSN: 1435-0661
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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  • 10
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    Chemical Processes in Soils (2006)
    Wiley
    Details
    Publication Date: 2006-03-01
    Print ISSN: 0361-5995
    Electronic ISSN: 1435-0661
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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