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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 4 (1964), S. 48-55 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Summary Phosphate liberating enzymes are demonstrated by a simultaneous heavy metal method whereby lead complexes are used. The advantages of using lead complexes are described: In the alkaline range one reaction stage can be left out in contrast to the Gomori method. In the neutral and acid range no disturbances occur through unspezific deposits despite relatively high concentrations of lead and organic phosphates. Instructions for the histochemical demonstration of six enzymes which liberate anorganic phosphate are given as examples and illustrated.
    Notes: Zusammenfassung Phosphatfreisetzende Enzyme werden histochemisch nach einer Simultan-methode dargestellt, bei welcher Bleikomplexe verwendet werden. Die Vorteile der Verwendung von Bleikomplexen werden aufgeführt: Im alkalischen Bereich kann gegenüber den Gomori-Methoden ein Reaktionsschritt eingespart werden, im neutralen und sauren Bereich treten trotz relativ hoher Blei -und Organophosphatkonzentrationen keine Störungen durch unspezifische Niederschläge auf. Vorschriften zur histochemischen Darstellung von sechs phosphatfreisetzenden Enzymen werden als Beispiele aufgeführt und mit Abbildungen belegt.
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  • 2
    ISSN: 1573-4927
    Keywords: esterases ; mouse genetics ; Es-17
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Genetic variation of a new codominantly inherited esterase, designated ES-17, has been discovered in the house mouse using isoelectric focusing in polyacrylamide gels. The ES-17 A phenotype (three bands; isoelectric points, between pH 5.55 and pH 5.90) was found in C57 BL/10Sn. LP/J possessed the Es-17B phenotype (three bands; isoelectric points, pH 5.05–5.55). ES-17 was present in all tissues examined, except for hemolysate and serum, and was most clearly expressed in the small intestine. Because of its reaction toward various substrates and inhibitors, ES-17 has tentatively been classified as acetyl esterase (EC 3.1.1.6). ES-17 was shown to be controlled by the structural locus Es-17, located on chromosome 9. From test-cross data, a gene order of Es-17-8.7±2.5 map units-Mpi-1-10.2±2.7 map units-Mod-1 was established.
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  • 3
    ISSN: 1573-4927
    Keywords: esterases ; mouse genetics ; Es-17
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Genetic variation of a new codominantly inherited esterase, designated ES-17, has been discovered in the house mouse using isoelectric focusing in polyacrylamide gels. The ES-17 A phenotype (three bands; isoelectric points, betweenpH 5.55 andpH 5.90) was found in C57 BL/10Sn. LP/J possessed the Es-17B phenotype (three bands; isoelectric points,pH 5.05–5.55). ES-17 was present in all tissues examined, except for hemolysate and serum, and was most clearly expressed in the small intestine. Because of its reaction toward various substrates and inhibitors, ES-17 has tentatively been classified as acetyl esterase (EC 3.1.1.6). ES-17 was shown to be controlled by the structural locusEs-17, located on chromosome 9. From test-cross data, a gene order ofEs-17-8.7±2.5 map units-Mpi-1-10.2±2.7 map units-Mod-1 was established.
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  • 4
    ISSN: 1573-4927
    Keywords: esterase-16 ; rat genetics ; linkage group V (LGV) ; cluster 2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Esterase-16, an esterase present in lung and other tissues of the laboratory rat, has been characterized by its biochemical properties (electrophoretic mobility, substrate pattern, sensitivity to inhibitors) and genetic variation in 107 inbred strains and substrains including 14 RI strains. It was classified as a carboxylesterase (EC 3.1.1.1). The phenotype ES-16A (BN/Han and 63 other strains) was defined as a narrow electrophoretic band migrating between ES-1A and ES-13A, ES-16B (LEW/Han and 42 other strains) exhibited the same electrophoretic mobility as ES-16A but was distinguished by its extremely weak activity. Segregation of ES-16 in RI strains and backcrosses indicated linkage to linkage group V (LGV). TheEs-16 locus was tentatively placed into esterase cluster 2 and homology withEs-7 of the house mouse is proposed.
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  • 5
    ISSN: 1573-4927
    Keywords: Mus musculus ; esterase ; Es-16 ; Chr. 3
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A polymorphism for an isozyme of a presumed arylesterase, esterase-16 (EC 3.1.1.2), has been detected in kidney, heart, and spleen of the house mouse, Mus musculus, by means of isoelectric focusing and by disc electrophoresis. Three phenotypes can be distinguished: the ES-16A phenotype (IEP 5.9) was found in C57BL/10Sn and many other laboratory inbred strains; the ES-16B phenotype (IEP 6.1) was found in M. m. molossinus; and the ES-16C phenotype (IEP 5.9; very weak activity) was found in Peru-Coppock. Esterase-16 is strongly inhibited by 10−3 m p-chloromercuribenzoate, but not by 2·10−4 m bis-p-nitrophenyl phosphate or by 10−3 m Diamox. It stains well with indoxyl acetate and other indigogenic substrates but only weakly with α-naphthyl acetate. Esterase-16 is completely insoluble in water. It is apparently governed by a structural gene locus, Es-16, with three alleles, Es-16 a , Es-16b, and Es-16 c, respectively. Es-16 is closely linked to Car-1 and Car-2 on chromosome 3. Typing of 94 animals of the backcross (C57BL/10Sn × M. m. mol.) F1 × M. m. mol. revealed a recombination frequency of 8.51±2.9%.
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  • 6
    ISSN: 1573-4927
    Keywords: arylesterase ; mouse genetics ; esterase-25 (ES-25) ; chromosome 12
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Genetic variation of a codominantly inherited kidney esterase, designated ES-25, has been discovered in the house mouse using disc electrophoresis. The ES-25A phenotype was found in A strains, AKR, and BALB/c. ES-25B was found in C57 BL strains and several other laboratory strains. The enzyme was shown to be controlled by a presumed structural locus,Es-25. The high concordance in 48 RI strains ofEs-25 withLy-18 indicated the location ofEs-25 on chromosome 12. The gene orderEs-25—Ly-18—D12Nyu1—Pre-1 was proposed.
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  • 7
    ISSN: 1573-4927
    Keywords: esterase-16 ; rat genetics ; linkage group V (LGV) ; cluster 2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Esterase-16, an esterase present in lung and other tissues of the laboratory rat, has been characterized by its biochemical properties (electrophoretic mobility, substrate pattern, sensitivity to inhibitors) and genetic variation in 107 inbred strains and substrains including 14 RI strains. It was classified as a carboxylesterase (EC 3.1.1.1). The phenotype ES-16A (BN/Han and 63 other strains) was defined as a narrow electrophoretic band migrating between ES-1A and ES-13A, ES-16B (LEW/Han and 42 other strains) exhibited the same electrophoretic mobility as ES-16A but was distinguished by its extremely weak activity. Segregation of ES-16 in RI strains and backcrosses indicated linkage to linkage group V (LGV). TheEs-16 locus was tentatively placed into esterase cluster 2 and homology withEs-7 of the house mouse is proposed.
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  • 8
    ISSN: 1573-4927
    Keywords: Mus musculus ; esterase-18 ; allozymes ; chromosome 19 ; gene order
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract This study describes the biochemical characterization, genetic variation, and linkage of a codominantly inherited murine esterase, termed ES-18. The enzyme was identified by isoelectric focusing of supernatants obtained after centrifugation of tissue homogenates and subsequent staining for esterase using either α-naphthyl acetate or 4-methylumbelliferyl elaidate as substrate. ES-18 exhibited an organ-specific variation of the intensity pattern of bands as seen in kidney, spleen, and macrophages, respectively. Its activity was highly sensitive to inhibition by 1 mmol · liter−1 p-chloromercuriphenyl-sulfonate but was resistant to bis-p-nitrophenyl phosphate. Four allozymes could be distinguished in kidney supernatants obtained from the inbred strains C57BL/10Sn (ES-18A), MOLF/Ei (ES-18B), WLL/BrA (ES-18C), and CAST/Ei (ES-18D). The enzyme is shown to be controlled by a structural locus,Es-18, which resides on chromosome 19. The gene orderLy-1—Got-1—4.7±1.6—Es-18 is suggested.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Biochemical genetics 20 (1982), S. 351-358 
    ISSN: 1573-4927
    Keywords: Mus musculus ; esterase-5 ; Esr locus ; chromosome 6
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Electrophoretic variation characterized by the presence (ES-5B+) or absence (ES-5B−) of esterase-5B in the plasma of the house mouse has been observed. It is suggested that the expression of esterase-5B is controlled by an autosomal locus, Esr, linked to Ldr-1 on chromosome 6, in addition to the presumptive structural locus Es-5, which is located on chromosome 8. A gene order of Lyt-3-Esr-Ldr-1 was determined by two crosses.
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  • 10
    ISSN: 1573-4927
    Keywords: esterase ; mouse genetics ; esterase-26 (ES-26) ; chromosome 3
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Genetic variation of a codominantly inherited esterase, designated ES-26, has been discovered in the house mouse using isoelectric focusing in polyacrylamide gels. The ES-26A phenotype (pI 8.2) was found in C57BL/10Sn. A/J showed the ES-26B phenotype (pI 7.8–7.9). A third phenotype, ES-26C (double-banded: pI's 8.1 and 8.3), was observed in SJL/J. ES-26 was detected only in liver, stomach, and small intestine. The enzyme was shown to be controlled by the presumed structural locus Es-26, located on chromosome 3. From a four-point cross, the gene order Car-2–6.2±2.7-Es-16–21.0±4.5-Es-26–13.6±3.8-Amy-1 was established.
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