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  • 1
    Electronic Resource
    Electronic Resource
    The need for genetically engineered food when enough is produced and unused (2001)
    Bradford : Emerald
    British food journal 103 (2001), S. 791-795 
    Details
    ISSN: 0007-070X
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Argues that, although the global production of food may be sufficient to feed the world's population, problems of poverty and distribution of food resources mean that hunger and malnutrition are still endemic in the developing world. In the face of these issues, the author suggests that biotechnology should be used to develop food crops whichoffer greater nutritional benefits so that the poorest people can obtain adequate nourishment from smaller quantities of food.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1108/00070700110696869
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  • 2
    Electronic Resource
    Electronic Resource
    Efficient fertile plant regeneration from protoplasts of the Indica rice breeding line IR72 (Oryza sativa L.) (1992)
    Springer
    Plant cell reports 11 (1992), S. 229-233 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Protoplasts were isolated from embryogenic cell suspensions obtained from mature seed derived embryogenic callus of the advanced Indica type rice breeding line IR72 available from IRRI (International Rice Research Institute), Manila. Culture of protoplasts with the agarose bead type method without nurse culture led to sustained proliferation of protoplast derived clones. A simple culture protocol was developed which stimulated embryogenic development. Germination of somatic embryos has so far produced 277 green plants from 6 independent experiments. 117 plants have been transferred to soil and are growing in the greenhouse. A few of them have already flowered and set seeds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00235071
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  • 3
    Electronic Resource
    Electronic Resource
    Gene transfer by electroporation into intact scutellum cells of wheat embryos (1993)
    Springer
    Plant cell reports 12 (1993), S. 671-675 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Gene transfer into intact cells was achieved by electroporating zygotic wheat embryos without any special pretreatment. Electroporation was tissue specific in so far as scutellum cells were found to be much more susceptible to gene transfer than other cell types of the embryo. The orientation of the embryos in the electroporation chamber also influenced the number of transformed scutellum cells; during electroporation, as in electrophoresis, the negatively charged plasmid DNA molecules seemed to move towards the positive electrode. Therefore, the embryos were arranged so that the scutella faced the negative electrode. The use of plasmids carrying either two chimeric anthocyanin regulatory genes or a chimeric gusA gene allowed clear identification of transformed cells in the scutellum. On some of the embryos, more than 100 transformed scutellum cells were found after electroporation with single electric pulses of 275 V/cm discharged from a 960-μF capacitor and with 100 μg DNA/ml electroporation buffer. Using the anthocyanin marker system, visibly transformed cells grew to produce red sectors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233417
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  • 4
    Electronic Resource
    Electronic Resource
    Fertile Indica rice plants regenerated from protoplasts isolated from microspore derived cell suspensions (1990)
    Springer
    Plant cell reports 9 (1990), S. 253-256 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rice plants (Oryza sativa L., Chinsurah Boro II var. Indica) were regenerated from protoplasts isolated from microspore derived cell suspensions. A simple procedure for the establishment of such cell suspension cultures from embryogenic microcallus derived from cultured isolated microspores of Indica-type rice is described. Regenerating protoplasts could readily be isolated from 5–12 months old cell suspensions showing visible colony formation in the range of 180–1050 colonies/106 protoplasts after about one month in culture. More than 100 independent green plantlets were regenerated via secondary embryogenesis from ca 20×106 protoplasts. Out of 32 plants grown to maturity under greenhouse conditions 24 were fertile.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232295
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  • 5
    Electronic Resource
    Electronic Resource
    Genetically Engineered Fertile Indica-Rice Recovered from Protoplasts (1990)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 8 (1990), S. 736-740 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] We have established an efficient protocol for plant regeneration from haploid Indica-type rice protoplasts. Incubation of these protoplasts with the selectable hygromycin phosphotransferase (hph) gene expressed under control of the 35S promoter of cauliflower mosaic virus (CaMV) and ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0890-736
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  • 6
    Electronic Resource
    Electronic Resource
    Genetic Engineering of Rice for Resistance to Sheath Blight (1995)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 13 (1995), S. 686-691 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] A 1.1 kb rice genomic DNA fragment, containing a chitinase gene under the control of the CaMV 35S promoter, was cloned into the rice transformation vector pGL2. After transformation of Indica rice protoplasts in the presence of polyethyleneglycol, plants were regenerated. The presence of the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0795-686
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  • 7
    Electronic Resource
    Electronic Resource
    In vitro clonal multiplication of mature trees of Dalbergia sissoo Roxb. (1982)
    Springer
    Plant cell, tissue and organ culture 2 (1982), S. 15-20 
    Details
    ISSN: 1573-5044
    Keywords: Dalbergia ; clonal multiplication ; nodal explants ; tissue culture ; elite trees
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Multiple shoots were obtained from nodal explants of 30-year-old trees of Dalbergia sissoo Roxb. on a defined medium, MS (Murashige & Skoog medium) supplemented with auxin-cytokinin combinations. IAA (Indole accetic acid) alone promoted 15% rooted shoot buds. A combination of IAA+Kn (Kinetin) gave 100% rooted shoot buds. A combination of NAA (napthaleneacetic acid) + Kn and NAA+BAP (6-benzylaminopurine) also gave high percentages of rooted shoot buds. Ascorbic acid in the medium prevented the death of callus and plantlets, which followed darkening of the medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033548
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  • 8
    Electronic Resource
    Electronic Resource
    Herbicide-resistant Indica rice plants from IRRI breeding line IR72 after PEG-mediated transformation of protoplasts (1992)
    Springer
    Plant molecular biology 20 (1992), S. 619-629 
    Details
    ISSN: 1573-5028
    Keywords: Indica rice ; IRRI breeding line ; PEG-mediated protoplast transformation ; hygromycin phosphotransferase ; phosphinothricin acetyltransferase ; herbicide resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The commercially important Indica rice cultivar Oryza sativa cv. IR72 has been transformed using direct gene transfer to protoplasts. PEG-mediated transformation was done with two plasmid constructs containing either a CaMV 35S promoter/HPH chimaeric gene conferring resistance to hygromycin (Hg) or a CaMV 35S promoter/BAR chimaeric gene conferring resistance to a commercial herbicide (Basta) containing phosphinothricin (PPT). We have obtained so far 92 Hgr and 170 PPTr IR72 plants from protoplasts through selection. 31 Hgr and 70 PPTr plants are being grown in the greenhouse to maturity. Data from Southern analysis and enzyme assays proved that the transgene was stably integrated into the host genome and expressed. Transgenic plants showed complete resistance to high doses of the commercial formulations of PPT.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00046447
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  • 9
    Electronic Resource
    Electronic Resource
    Structural defects and photosensitivity in evaporated lead selenide films (1982)
    Springer
    Journal of materials science 1 (1982), S. 83-86 
    Details
    ISSN: 1573-4811
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00731033
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  • 10
    Electronic Resource
    Electronic Resource
    Evidence for genomic changes in transgenic rice (Oryza sativa L.) recovered from protoplasts (1996)
    Springer
    Transgenic research 5 (1996), S. 97-103 
    Details
    ISSN: 1573-9368
    Keywords: Transgenic rice ; PCR-RAPD ; arbitrary DNA primers ; genomic changes ; somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The occurrence of genomic modifications in transgenic rice plants recovered from protoplasts and their transmission to the self-pollination progeny has been verfied with the random amplified polymorphic DNA (RAPD) approach. The plant was the Indica-type rice (Oryza sativa L.) cultivar Chinsurah Boro II. The analysed material was: (1) microspore-derived embryogenic rice cells grown in suspension culture, (2) transgenic plants recovered from protoplasts produced from the cultured cells and (3) the self-pollination progeny (two successive generations) of the transgenic plants. DNA purified from samples of these materials was PCR-amplified with different random oligonucleotide primers and the amplification products were analysed by agarose gel electrophoresis. Band polymorphism was scored and used in band-sharing analyses to produce a similarity matrix. Relationships among the analysed genomes were expressed in a dendrogram. The extensive DNA changes evidenced in cultured cells demonstrate the occurrence of somaclonal variation in the material used to produce protoplasts for gene transfer. Quantitatively reduced DNA changes were also found in the resulting transgenic plants and i their self-pollination progenies. While confirming the stability of the foreign gene in transgenic plants, this work gives molecular evidence for the occurrence of stable genomic changes in transgenic plants and points toin vitro cell culture as the causative agent. RAPDs are shown to be a convenient tool to detect and estimate the phenomenon at the molecular level. The methodology is also proposed as a fast tool to select those transgenic individuals that retain the most balanced genomic structure and to control the result of back-crosses planned to restore the original genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01969427
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