ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
LuxS of Bacillus subtilis is a member of a novel family of proteins with a potential role in quorum sensing, controlling important aspects of cellular physiology in a range of microbial species. B. subtilis luxS was cloned, expressed in Escherichia coli, purified and crystallized using the hanging-drop method of vapour diffusion with ammonium sulfate as the precipitant. The crystals belong to one of the enantiomorphic space groups P6122 or P6522, with approximate unit-cell parameters a = b = 63.6, c = 151.5 Å and one subunit in the asymmetric unit, corresponding to a packing density of 2.5 Å3 Da−1. The crystals diffract X-rays to at least 1.55 Å resolution on a synchrotron-radiation source. Determination of the structure will provide insights into the key determinants of function of this class of proteins, for which no structures are currently available.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444901011611
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