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Vital and Calcofluor staining ofCosmarium and its protoplasts (1978)

Berliner, Martha D. ; Wood, Nancy L. ; Damico, Josephine

Springer

Protoplasma 96 (1978), S. 39-46

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ISSN: 1615-6102

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary Intact cells, protoplasts, primary and secondary walls ofCosmarium species were stained with 13 vital stains and with Calcofluor. The intracellular contents of viable cells and protoplasts were not stained with any of the test dyes although the primary and secondary walls often were stained. Protoplasts disintegrated after short periods in acid stains but did survive for up to 24 hours in several dilute basic dyes. Once the protoplast membrane was damaged, the nuclei and cytoplasm were brightly stained with most dyes, whereas the vacuoles remained unstained. Calcofluor was particularly useful in determining the porous structure and pattern of the primary walls.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01279573

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