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  • 1
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; Triticum turgidum ; Hordeum vulgare ; barley ; chemotypes ; electrophoresis ; variation ; prolamines ; gliadins ; hordeins ; electrophoregram ; genetic resources
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Polyacrylamide gel electrophoresis (PAGE) of storage proteins (prolamines) was used to screen 64 landraces of wheat and barley from Nepal and the YemenArab Republic and two cultivars for comparison. Altogether 3168 single seeds were examined and the advantages gained by using the vertical slab gel method were recognised. The extent of variation present within populations of landraces could be assessed easily and rapidly using the methods described. Differences in ploidy levels of wheats were detected by PAGE and investigated. Suggestions are made for improvements in sampling strategies in hilly terrain.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Genetic resources and crop evolution 39 (1992), S. 97-105 
    ISSN: 1573-5109
    Keywords: dryland conditions ; durum wheat ; gene pool ; germplasm ; kernel ; spike
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A set of 109 durum wheat accessions, selected from a world collection for their adaptation to dry environments, were further evaluated under moisture-limiting conditions. The entries were subdivided into 9 gene pools based on their country of origin. Data are presented to assess the phenotypic variation and distinctiveness of these pools for some spike and grain features. Analysis of variance revealed highly significant differences among materials of diverse origin for all traits except protein content. Within-country variation was generally wide as regards spike length and 1000-kernel weight. The Ethiopian germplasm presented a remarkable variability and distinctiveness. Entries with interesting values for breeding purposes were detected in most country gene pools. In general, selection of germplasm adapted to harsh environments did not result in a loss of variability for the considered traits, with the possible exception of protein content. Ethiopian entries presented the highest number of significant pairwise character correlations. The two most important traits in a discriminant analysis by country of origin were spike density and 1000-kernel weight. Diversity among gene pools was further assessed by a canonical analysis. The first three canonical variables explained 90% of the total variance. The gene pools of Ethiopia, Greece, Egypt and Jordan appeared clearly distinct from the other germplasm subsets studied. Geographically distinct patterns emerging from this study could be the result of the combined effect of natural and human selection.
    Type of Medium: Electronic Resource
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