ISSN:
0021-9304
Keywords:
epithelium
;
migration
;
topography
;
porosity
;
corneal epithelium
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
,
Technology
Notes:
Corneal epithelial tissue migration over the surface of a synthetic polymer can be inhibited by pores in the substrate. The effects of this substrate topography upon epithelial tissue migration were studied in vitro. Membranes of different porosities and structures were used to provide two series of surfaces having a graded increase in discontinuities: cellulose nitrate/acetate membranes with a tortuous network of pores, and track-etched polycarbonate membranes with columnar pores. Corneal epithelial tissue outgrowth was inhibited by increased pore size, and for both series of membranes, outgrowth was completely halted on membranes with mean diameter of the pores 0.9 μm at the pore densities measured. On the track-etched membranes with pores of 〈0.9 μm diameter, tissue outgrowth could be partially “rescued” by coating with fibronectin or collagen, but above this size, the inhibition predominated. The effect of porosity of the track-etched membranes upon the migration of dissociated epithelial cells was also examined. Although migration of these cells was reduced on membranes having pore sizes larger than 0.9 μm, it was not completely inhibited even on membranes of 2.3-μm pore diameter. Therefore, tissue movement of adult stratified epithelium may be inhibited by specific surface topographies, and in this assay system, epithelial tissue outgrowth was more affected than was the migration of dissociated epithelial cells. © 1998 John Wiley & Sons, Inc. J Biomed Mater Res, 42, 245-257, 1998.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
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