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  • 1
    Electronic Resource
    Electronic Resource
    Development of an enzyme-linked immunosorbent assay for detection of antibodies to Babesia bigemina in cattle (1998)
    Springer
    Parasitology research 84 (1998), S. 651-656 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Monoclonal antibodies, directed against a 58-kDa Babesia bigemina merozoite antigen that reacted strongly with immune sera from experimentally and naturally infected cattle in Western blots, were used to develop a competitive-inhibition enzyme-linked immunosorbent assay (ELISA). As based on the testing of 70 antibody-positive sera from experimentally infected cattle and 166 antibody-negative sera collected in non-endemic areas of Australia, the sensitivity and specificity of the ELISA were 95.7% and 97.0%, respectively. In sequential sera collected from six calves during the course of experimental B. bigemina infections the ELISA detected seroconversion at about 10 days post-inoculation. The specificity of the ELISA was not affected by the presence of antibodies to B. bovis, Anaplasma marginale or Theileria buffeli. In 42 sera from cattle experimentally infected with B. bovis but negative for B. bigemina the specificity of the ELISA was 95.2%. The use of a competitive-inhibition ELISA format detecting only antibody directed against a single epitope on the 58-kDa antigen appears to have overcome many of the specificity problems that have plagued serological tests for B. bigemina in the past. The test should be useful for epidemiology studies, particularly in areas where B. bovis and B. bigemina have overlapping distributions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004360050465
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  • 2
    Electronic Resource
    Electronic Resource
    Reduction in pathogenicity ofBabesia bovis for its tick vector,Boophilus microplus, after rapid blood passage in splenectomized calves (1981)
    Springer
    Parasitology research 64 (1981), S. 347-351 
    Details
    ISSN: 1432-1955
    Keywords: Babesia bovis ; Babesia pathogenicity for tick vector ; Boophilus microplus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Two strains ofBabesia bovis that had been serially blood passaged in splenectomized calves 27 to 33 times, a procedure known to have reduced their virulence for normal cattle, were shown to have low pathogenicity for replete, femaleBoophilus microplus. In comparison with a strain ofB. bovis unmodified by repeated blood passage, the two modified strains infected higher proportions of ticks and produced comparable numbers of morphologically similar parasites in their haemolymph, but killed significantly fewer of them. Red discolouration of haemolymph was observed in many ticks infected with the unmodified strain, but in none of those infected with the modified strains. It is suggested that the modified strains have lost a quality causing pathological effects on the gut cells of infected ticks.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00927381
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    Paper (German National Licenses)
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