Publication Date:
1988-12-09
Description:
Two genome rearrangements involving 11- and 55-kilobase DNA elements occur during the terminal differentiation of an Anabaena photosynthetic vegetative cell into a nitrogen-fixing heterocyst. The xisA gene, located on the nifD 11-kilobase DNA element, was inactivated by recombination between the chromosome and a copy of the xisA gene that was mutated by inserting an antibiotic gene cassette. Site-directed inactivation of the Anabaena xisA gene blocked rearrangement of the 11-kilobase element and nitrogen fixation, but did not affect rearrangement of the 55-kilobase element, heterocyst differentiation, or heterocyst pattern formation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Golden, J W -- Wiest, D R -- GM36890/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1988 Dec 9;242(4884):1421-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biology, Texas A&M University, College Station 77843.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3144039" target="_blank"〉PubMed〈/a〉
Keywords:
Cyanobacteria/*genetics/growth & development
;
DNA/genetics
;
*Gene Rearrangement
;
*Genes
;
*Genes, Bacterial
;
Immunoblotting
;
Nitrogen Fixation/*genetics
;
Phenotype
;
Restriction Mapping
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink
