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  • 1
    Publication Date: 2011-08-19
    Description: Author(s): D. R. Stevens, E. W. Skau, L. N. Downen, M. P. Roman, and L. I. Clarke Monte Carlo simulations of finite-size effects for continuum percolation in three-dimensional, rectangular sample spaces filled with spherical particles were performed. For samples with any dimension less than 10–20 times the particle diameter, finite-size effects were observed. For thin films in th... [Phys. Rev. E 84, 021126] Published Thu Aug 18, 2011
    Keywords: Statistical physics
    Print ISSN: 1539-3755
    Electronic ISSN: 1550-2376
    Topics: Physics
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  • 2
    Publication Date: 2001-11-27
    Description: Adhesions between fibroblastic cells and extracellular matrix have been studied extensively in vitro, but little is known about their in vivo counterparts. Here, we characterized the composition and function of adhesions in three-dimensional (3D) matrices derived from tissues or cell culture. "3D-matrix adhesions" differ from focal and fibrillar adhesions characterized on 2D substrates in their content of alpha5beta1 and alphavbeta3 integrins, paxillin, other cytoskeletal components, and tyrosine phosphorylation of focal adhesion kinase (FAK). Relative to 2D substrates, 3D-matrix interactions also display enhanced cell biological activities and narrowed integrin usage. These distinctive in vivo 3D-matrix adhesions differ in structure, localization, and function from classically described in vitro adhesions, and as such they may be more biologically relevant to living organisms.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cukierman, E -- Pankov, R -- Stevens, D R -- Yamada, K M -- New York, N.Y. -- Science. 2001 Nov 23;294(5547):1708-12.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11721053" target="_blank"〉PubMed〈/a〉
    Keywords: 3T3 Cells ; Animals ; *Cell Adhesion/drug effects ; Cell Culture Techniques/methods ; Cell Division ; Cell Movement ; Cell Size ; Cells, Cultured ; Culture Techniques/methods ; Cycloheximide/pharmacology ; Cytoskeletal Proteins/metabolism ; Extracellular Matrix/chemistry/metabolism ; Fibroblasts/chemistry/*cytology/*metabolism ; Fibronectins/metabolism ; Fluorescent Antibody Technique, Indirect ; Focal Adhesion Kinase 1 ; Focal Adhesion Protein-Tyrosine Kinases ; Focal Adhesions/chemistry/metabolism ; Glutaral/metabolism ; Humans ; Imaging, Three-Dimensional/*methods ; Integrins/metabolism ; Mice ; Mitogen-Activated Protein Kinases/metabolism ; Molecular Conformation ; Phosphorylation ; Protein-Tyrosine Kinases/metabolism ; Time Factors
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 3
    Publication Date: 1979-09-28
    Description: The opiate antagonist naloxone precipitates withdrawal when given either 15 minutes after or 1 minute before a single injection of morphine in drug-naive mice. We propose that withdrawal signs arise from a synergistic mixture of excitatory influences that are direct (agonistic action on nonspecific opiate receptors) and indirect (sensory and affective disorders, stress, hormonal and neurotransmitter dysfunction, and so forth). The predominant effects during precipitated withdrawal are assumed to be direct, whereas during abstinence in tolerant animals they are indirect.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Stevens, D R -- Klemm, W R -- New York, N.Y. -- Science. 1979 Sep 28;205(4413):1379-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/224462" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Behavior, Animal/physiology ; Dose-Response Relationship, Drug ; Drug Interactions ; Drug Tolerance ; Female ; Humans ; Mice ; Morphine/*pharmacology ; Naloxone/*pharmacology ; Receptors, Opioid/*drug effects ; Stereotyped Behavior/physiology ; Substance Withdrawal Syndrome/*physiopathology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 251 (1996), S. 23-30 
    ISSN: 1617-4623
    Keywords: Chlamydomonas ; Transformation ; Dominant marker ; ble
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A chimeric gene composed of the coding sequence of theble gene fromStreptoalloteichus hindustanus fused to the 5′ and 3′ untranslated regions of theChlamydomonas reinhardtii nuclear geneRBCS2 has been constructed. Introduction of this chimeric gene into the nuclear genome ofC. reinhardtii by co-transformation with theARG7 marker yields Arg+ transformants of which approximately 80% possess theble gene. Of these co-transformants, approximately 3% display a phleomycin-resistant (PmR) phenotype. Western blot analysis using antibodies against theble gene product confirms the presence of the protein in the PmR transformants and genetic analysis demonstrates the co-segregation of theble gene with the phenotype in progeny arising from the mating of a PmR transformant to wild-type strains. Direct selection of PmR transformants was achieved by allowing an 18-h period for recovery and growth of transformed cells prior to selection. This work represents the first demonstration of stable expression and inheritance of a foreign gene in the nuclear genome ofC. reinhardtii and provides a useful dominant marker for nuclear transformation.
    Type of Medium: Electronic Resource
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  • 5
    Publication Date: 2008-12-19
    Print ISSN: 1098-0121
    Electronic ISSN: 1550-235X
    Topics: Physics
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  • 6
    Publication Date: 2012-10-01
    Print ISSN: 0034-6748
    Electronic ISSN: 1089-7623
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
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  • 7
    Publication Date: 2016-09-30
    Description: Dissolved organic matter (DOM) quality and quantity is not measured routinely in-situ limiting our ability to quantify DOM process dynamics. This is problematic given legislative obligations to determine event based variability, however, recent advances in field deployable optical sensing technology provide the opportunity to address this problem. In this paper we outline a new approach for in-situ quantification of DOM quantity (Dissolved Organic Carbon: DOC) and a component of quality (Biochemical Oxygen Demand: BOD) using a multi-wavelength, through-flow fluorescence sensor. The sensor measured tryptophan-like (Peak T) and humic-like (Peak C) fluorescence, alongside water temperature and turbidity. Laboratory derived coefficients were developed to compensate for thermal quenching and turbidity interference (i.e. light attenuation and scattering). Field tests were undertaken on an urban river with ageing wastewater and stormwater infrastructure (Bourn Brook; Birmingham, UK). Sensor output was validated against laboratory determinations of DOC and BOD collected by discrete grab sampling during baseflow and stormflow conditions. Data driven regression models were then compared to laboratory correction methods. A combination of temperature and turbidity compensated Peak T and Peak C was found to be a good predictor of DOC concentration (R 2  = 0.92). Conversely, using temperature and turbidity correction coefficients provided low predictive power for BOD (R 2  = 0.46 and R 2  = 0.51, for Peak C and T respectively). For this study system, turbidity appeared to be a reasonable proxy for BOD, R 2  = 0.86. However, a linear mixed effect model with temperature compensated Peak T and turbidity provided a robust BOD prediction (R 2  = 0.95). These findings indicate that with careful initial calibration, multi-wavelength fluorescence, coupled with turbidity and temperature provides a feasible proxy for continuous, in-situ measurement of DOC concentration and BOD. This approach represents a cost effective monitoring solution, particularly when compared to UV- absorbance sensors and DOC analysers, and could be readily adopted for research and industrial applications.This article is protected by copyright. All rights reserved.
    Print ISSN: 0885-6087
    Electronic ISSN: 1099-1085
    Topics: Architecture, Civil Engineering, Surveying , Geography
    Published by Wiley
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  • 8
    Publication Date: 2011-08-18
    Print ISSN: 1539-3755
    Electronic ISSN: 1550-2376
    Topics: Physics
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  • 9
    Publication Date: 2006-06-01
    Print ISSN: 0266-0032
    Electronic ISSN: 1475-2743
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley on behalf of British Society of Soil Science.
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  • 10
    Publication Date: 2009-03-03
    Description: SUMMARYA model of the growth, pregnancy and lactation of red deer was developed for incorporation into a whole-farm systems model in order to improve the understanding of venison supply systems. The model estimates the level of metabolic demand for a deer, which depends on the maximum capacity of its tissues to use energy. A function that takes account of satiation signals and rumen capacity is used to convert the metabolic demand into an estimate of the deer's forage intake demand, which can be used as an input into a foraging model. The actual energy intake of the deer is subsequently used to predict live weight (LW), body condition score, foetal growth and gestation length in pregnant hinds, and milk yield in lactating hinds. In order to make these predictions, the model requires inputs that include values for mean daily temperature, mean daily wind speed, day length and season, as well as pasture quality. Values for model parameters were obtained from the literature, rather than by fitting to data, and model predictions were then compared with measurements obtained in independent trials.In simulations, the model predicted that 152-day-old stags and hinds, weighing, respectively, 44 and 48 kg, would grow to, respectively, 106 and 90 kg when 517 days old, compared with trial results of, respectively, 103 and 84 kg. Predictions for the weight of pregnant hinds, gestation length and calf birth weight compared well with an experiment for hinds on a high plane of nutrition but poorly for hinds on medium and low planes. Weekly predictions of hind LWs for days 132–230 of pregnancy had respective residual means of 0·08, 6·2 and 8·5 kg, and respective residual standard deviations of 1·33, 4·6 and 5·2 kg for the high, medium and low nutritional planes. Predicted gestation length for high, medium and low planes of nutrition were, respectively, 231·5, 238·0 and 242·0 days compared with experimental values of, respectively, 231·3, 234·7 and 239·2 days, while predicted birth weights were, respectively, 8·5, 8·3 and 8·9 kg compared with measured values of, respectively, 8·4, 9·5 and 9·3 kg. Predicted calf growth from birth to 14 weeks agreed well with data (residual mean and standard deviation being 0·04 and 1·15 kg, respectively).The existing software structure of the whole-farm model dictated that the deer model use the Euler method with a fixed, daily time step. Therefore, the model was constructed using difference (rather than differential) equations and used a traditional, energy-balance method for predicting growth. This empirical approach tacitly imposed a standard body composition and standard metabolic rate for adults, with values corresponding to well-fed deer. This does not cater for variation in body composition and metabolic activity, and in retrospect, caused the model to perform poorly for the medium and low nutritional regimes.
    Print ISSN: 0021-8596
    Electronic ISSN: 1469-5146
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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