Publication Date:
2002-04-20
Description:
Simian virus 40 (SV40) utilizes endocytosis through caveolae for infectious entry into host cells. We found that after binding to caveolae, virus particles induced transient breakdown of actin stress fibers. Actin was then recruited to virus-loaded caveolae as actin patches that served as sites for actin "tail" formation. Dynamin II was also transiently recruited. These events depended on the presence of cholesterol and on the activation of tyrosine kinases that phosphorylated proteins in caveolae. They were necessary for formation of caveolae-derived endocytic vesicles and for infection of the cell. Thus, caveolar endocytosis is ligand-triggered and involves extensive rearrangement of the actin cytoskeleton.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Pelkmans, Lucas -- Puntener, Daniel -- Helenius, Ari -- New York, N.Y. -- Science. 2002 Apr 19;296(5567):535-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Swiss Federal Institute of Technology Zurich (ETHZ), HPM1 Building, ETH Honggerberg, CH-8093 Zurich, Switzerland.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11964480" target="_blank"〉PubMed〈/a〉
Keywords:
Actin Cytoskeleton/*physiology/ultrastructure
;
Actins/*metabolism
;
Animals
;
Bicyclo Compounds, Heterocyclic/pharmacology
;
Caveolae/*metabolism/ultrastructure/virology
;
Caveolin 1
;
Caveolins/metabolism
;
Cell Line
;
Cholesterol/physiology
;
*Depsipeptides
;
Dynamins
;
*Endocytosis
;
GTP Phosphohydrolases/genetics/*metabolism
;
Haplorhini
;
Peptides, Cyclic/pharmacology
;
Phosphorylation
;
Phosphotyrosine/metabolism
;
Protein-Tyrosine Kinases/antagonists & inhibitors/metabolism
;
Recombinant Fusion Proteins/metabolism
;
Simian virus 40/*physiology
;
Stress Fibers/metabolism
;
Thiazoles/pharmacology
;
Thiazolidines
;
Transport Vesicles/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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