Publication Date:
2002-06-29
Description:
Phototaxis and photophobic responses of green algae are mediated by rhodopsins with microbial-type chromophores. We report a complementary DNA sequence in the green alga Chlamydomonas reinhardtii that encodes a microbial opsin-related protein, which we term Channelopsin-1. The hydrophobic core region of the protein shows homology to the light-activated proton pump bacteriorhodopsin. Expression of Channelopsin-1, or only the hydrophobic core, in Xenopus laevis oocytes in the presence of all-trans retinal produces a light-gated conductance that shows characteristics of a channel selectively permeable for protons. We suggest that Channelrhodopsins are involved in phototaxis of green algae.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Nagel, Georg -- Ollig, Doris -- Fuhrmann, Markus -- Kateriya, Suneel -- Musti, Anna Maria -- Bamberg, Ernst -- Hegemann, Peter -- New York, N.Y. -- Science. 2002 Jun 28;296(5577):2395-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Max-Planck-Institut fur Biophysik, Kennedyallee 70, 60596 Frankfurt am Main, Germany. nagel@mpibp-frankfurt.mpg.de〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12089443" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Bacteriorhodopsins/chemistry/metabolism
;
Butyric Acid/pharmacology
;
Chlamydomonas reinhardtii/chemistry/genetics/*metabolism
;
Electric Conductivity
;
Hydrogen-Ion Concentration
;
Ion Channel Gating
;
Ion Channels/*chemistry/genetics/*metabolism
;
Ion Transport
;
*Light
;
Membrane Potentials
;
Molecular Sequence Data
;
Oocytes
;
Patch-Clamp Techniques
;
*Protons
;
RNA, Complementary
;
Recombinant Proteins/metabolism
;
Retinaldehyde/pharmacology
;
Sequence Alignment
;
Temperature
;
Xenopus laevis
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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