Publication Date:
2008-03-29
Description:
The synaptic response waveform, which determines signal integration properties in the brain, depends on the spatiotemporal profile of neurotransmitter in the synaptic cleft. Here, we show that electrophoretic interactions between AMPA receptor-mediated excitatory currents and negatively charged glutamate molecules accelerate the clearance of glutamate from the synaptic cleft, speeding up synaptic responses. This phenomenon is reversed upon depolarization and diminished when intracleft electric fields are weakened through a decrease in the AMPA receptor density. In contrast, the kinetics of receptor-mediated currents evoked by direct application of glutamate are voltage-independent, as are synaptic currents mediated by the electrically neutral neurotransmitter GABA. Voltage-dependent temporal tuning of excitatory synaptic responses may thus contribute to signal integration in neural circuits.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2685065/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2685065/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sylantyev, Sergiy -- Savtchenko, Leonid P -- Niu, Yin-Ping -- Ivanov, Anton I -- Jensen, Thomas P -- Kullmann, Dimitri M -- Xiao, Min-Yi -- Rusakov, Dmitri A -- 071179/Wellcome Trust/United Kingdom -- G0400627/Medical Research Council/United Kingdom -- G0400627(71256)/Medical Research Council/United Kingdom -- G0400627(76527)/Medical Research Council/United Kingdom -- G0600368/Medical Research Council/United Kingdom -- G0600368(77987)/Medical Research Council/United Kingdom -- G116/147/Medical Research Council/United Kingdom -- Medical Research Council/United Kingdom -- Wellcome Trust/United Kingdom -- New York, N.Y. -- Science. 2008 Mar 28;319(5871):1845-9. doi: 10.1126/science.1154330.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute of Neurology, University College London, Queen Square, London, WC1N 3BG, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18369150" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cells, Cultured
;
Dendrites/physiology
;
Diffusion
;
Dipeptides/pharmacology
;
*Excitatory Postsynaptic Potentials
;
Glutamic Acid/*metabolism
;
Magnesium/pharmacology
;
Male
;
Monte Carlo Method
;
Patch-Clamp Techniques
;
Pyramidal Cells/*physiology
;
Quinoxalines/pharmacology
;
Rats
;
Rats, Sprague-Dawley
;
Receptors, AMPA/antagonists & inhibitors/*metabolism
;
Receptors, GABA/metabolism
;
Synapses/*physiology
;
gamma-Aminobutyric Acid/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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