Publication Date:
2013-03-19
Description:
Embryonic stem (ES) cells are pluripotent and characterized by open chromatin and high transcription levels, achieved through auto-regulatory and feed-forward transcription factor loops. ES-cell identity is maintained by a core of factors including Oct4 (also known as Pou5f1), Sox2, Klf4, c-Myc (OSKM) and Nanog, and forced expression of the OSKM factors can reprogram somatic cells into induced pluripotent stem cells (iPSCs) resembling ES cells. These gene-specific factors for RNA-polymerase-II-mediated transcription recruit transcriptional cofactors and chromatin regulators that control access to and activity of the basal transcription machinery on gene promoters. How the basal transcription machinery is involved in setting and maintaining the pluripotent state is unclear. Here we show that knockdown of the transcription factor IID (TFIID) complex affects the pluripotent circuitry in mouse ES cells and inhibits reprogramming of fibroblasts. TFIID subunits and the OSKM factors form a feed-forward loop to induce and maintain a stable transcription state. Notably, transient expression of TFIID subunits greatly enhanced reprogramming. These results show that TFIID is critical for transcription-factor-mediated reprogramming. We anticipate that, by creating plasticity in gene expression programs, transcription complexes such as TFIID assist reprogramming into different cellular states.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Pijnappel, W W M Pim -- Esch, Daniel -- Baltissen, Marijke P A -- Wu, Guangming -- Mischerikow, Nikolai -- Bergsma, Atze J -- van der Wal, Erik -- Han, Dong Wook -- Bruch, Hermann vom -- Moritz, Soren -- Lijnzaad, Phillip -- Altelaar, A F Maarten -- Sameith, Katrin -- Zaehres, Holm -- Heck, Albert J R -- Holstege, Frank C P -- Scholer, Hans R -- Timmers, H T Marc -- England -- Nature. 2013 Mar 28;495(7442):516-9. doi: 10.1038/nature11970. Epub 2013 Mar 17.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Molecular Cancer Research, University Medical Center Utrecht, 3584 CG Utrecht, The Netherlands.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23503660" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cellular Reprogramming/genetics
;
Chromatin/genetics/metabolism
;
Embryonic Stem Cells/cytology/metabolism
;
Female
;
Fibroblasts/cytology/metabolism
;
Humans
;
Induced Pluripotent Stem Cells/cytology/metabolism
;
Male
;
Mice
;
Pluripotent Stem Cells/cytology/*metabolism
;
Promoter Regions, Genetic/genetics
;
RNA Polymerase II/metabolism
;
TATA-Binding Protein Associated Factors/deficiency/genetics/metabolism
;
TATA-Box Binding Protein/metabolism
;
Transcription Factor TFIID/deficiency/genetics/*metabolism
;
Transcription Factors/genetics/metabolism
;
*Transcription, Genetic
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink
