Publication Date:
1985-03-22
Description:
Guinea pigs were vaccinated with truncated herpes simplex virus type-1 (HSV-1) glycoprotein D produced in the genetically engineered mammalian cell line gD10.2. Vaccinated animals formed antibodies that neutralized both HSV-1 and herpes simplex virus type 2 (HSV-2) in an in vitro neutralization assay. Vaccinated animals were challenged with HSV-2 by intravaginal infection. Animals that received the immunogen in Freund's complete adjuvant were completely protected from the clinical manifestations of genital HSV-2 infection. Animals that received the immunogen incorporated in alum adjuvants were partly protected from clinical disease; the infections that did develop were significantly less severe than those that occurred in control animals injected with adjuvant alone. The results demonstrate that immunization with a purified viral protein can provide significant protection against primary genital infection by HSV-2 in guinea pigs.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Berman, P W -- Gregory, T -- Crase, D -- Lasky, L A -- New York, N.Y. -- Science. 1985 Mar 22;227(4693):1490-2.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2983428" target="_blank"〉PubMed〈/a〉
Keywords:
Adjuvants, Immunologic
;
*Aluminum Compounds
;
Aluminum Hydroxide
;
Animals
;
Antibodies, Viral/biosynthesis
;
Cloning, Molecular
;
Female
;
Freund's Adjuvant
;
Guinea Pigs
;
Herpes Genitalis/*prevention & control
;
Male
;
Neutralization Tests
;
Phosphates
;
Simplexvirus/*immunology
;
Vaccination
;
*Viral Envelope Proteins
;
Viral Proteins/genetics/*immunology
;
*Viral Vaccines/immunology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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