Publication Date:
2004-09-14
Description:
Siderophores are extracellular iron-binding compounds that mediate iron transport into many cells. We present evidence of analogous molecules for copper transport from methane-oxidizing bacteria, represented here by a small fluorescent chromopeptide (C45N12O14H62Cu, 1216 daltons) produced by Methylosinus trichosporium OB3b. The crystal structure of this compound, methanobactin, was resolved to 1.15 angstroms. It is composed of a tetrapeptide, a tripeptide, and several unusual moieties, including two 4-thionyl-5-hydroxy-imidazole chromophores that coordinate the copper, a pyrrolidine that confers a bend in the overall chain, and an amino-terminal isopropylester group. The copper coordination environment includes a dual nitrogen- and sulfur-donating system derived from the thionyl imidazolate moieties. Structural elucidation of this molecule has broad implications in terms of organo-copper chemistry, biological methane oxidation, and global carbon cycling.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kim, Hyung J -- Graham, David W -- DiSpirito, Alan A -- Alterman, Michail A -- Galeva, Nadezhda -- Larive, Cynthia K -- Asunskis, Dan -- Sherwood, Peter M A -- New York, N.Y. -- Science. 2004 Sep 10;305(5690):1612-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Civil, Environmental, and Architectural Engineering, University of Kansas, Lawrence, KS 66045, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15361623" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acids/analysis
;
Chemistry, Physical
;
Copper/analysis/chemistry/*metabolism
;
Crystallization
;
Crystallography, X-Ray
;
Dimerization
;
Imidazoles/*chemistry/isolation & purification/metabolism
;
Ligands
;
Methane/metabolism
;
Methylosinus trichosporium/chemistry/*metabolism
;
Models, Molecular
;
Molecular Structure
;
Molecular Weight
;
Oligopeptides/*chemistry/isolation & purification/metabolism
;
Oxidation-Reduction
;
Physicochemical Phenomena
;
Spectrum Analysis
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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