ISSN:
0006-3592
Keywords:
thyroid cells
;
immobilization
;
photografting
;
photocatalyst
;
thryrotrophin bioassay
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Viable and functionally responsive human thyroid follicular cells, suspended in a commercial polyester acrylate diluted with tripropyleneglycol diacrylate, photoinitiated, and photocatalyzed with a proprietary photocatalytic system based on a synergic mixture of vanadium (V) t-butoxide and i-propoxide, have been immobilized as monolayers onto polystyrene plates. Bioassay of thyrotrophin in immobilized cell cultures yielded, by log-log plot of the dose-response curve, a slope (0.92 ± 0.02) in close agreement with that (0.91) reported for cells immobilized by physical adsorption. The decisive role of photocatalyst in the photografting procedure has also been shown experimentally. A mechanism is suggested by which cells are anchored, rapidly and with stable chemical bonds, onto one of the two acrylate functions of the monomer-prepolymer mixture, the other one being simultaneously responsible for photochemical grafting onto the support. © 1993 John Wiley & Sons, Inc.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260420215
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