Publication Date:
2005-11-16
Description:
Background: Patients with chronic phase CML who achieve a complete cytogenetic response (CCR) have a low risk of disease progression. Since patients unlikely to achieve a CCR may benefit from more aggressive therapy, it would be clinically advantageous to identify those patients prior to therapy. Based on the hypothesis that cytogenetic refractoriness may be a property of leukemic progenitor cells, we explored the potential of gene expression profiling of CD34+ cells as a tool for predicting failure to achieve CCR. Methods: Fifty-one patients with CML who either achieved a CCR within 1 year of imatinib therapy (R, n = 35), or remained at least 65% Ph+ (NR, n = 16) were included in the study. Pre-imatinib CD34+ cells were FACS isolated from cryopreserved bone marrow mononuclear cells. RNA was extracted from the CD34+ cells and samples with 〉=5ng of high quality total RNA were amplified and labelled with the Affymetrix two cycle cDNA synthesis and IVT labeling protocol using
Print ISSN:
0006-4971
Electronic ISSN:
1528-0020
Topics:
Biology
,
Medicine
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