ISSN:
1432-0703
Source:
Springer Online Journal Archives 1860-2000
Topics:
Energy, Environment Protection, Nuclear Power Engineering
,
Medicine
Notes:
Abstract Rainbow trout (Salmo gairdneri R.) were force-fed 0.1 mCi (0.5 mg)3H-dodecylcyclohexane and sacrificed at 12, 24, 48 hr and 1 week after feeding in order to study the metabolic utilization of a naphthenic hydrocarbon. One week after dosing, approximately 25% of the ingested radioactivity was stored in the carcass and 3/4 of this radioactivity was due to unchanged hydrocarbon. In the liver, only 25% of the3H present after one week was associated with hydrocarbon. The incorporation of radioactivity in hepatic lipids 24 hr after ingestion of3H-dodecylcyclohexane revealed that radioactivity was equally incorporated into the phospholipids and neutral lipids. In neutral lipids, the free fatty acids were the most labeled fraction, whereas in phospholipids the greater deposition of radioactivity occurred in phosphatidyl choline. The major biotransformation products were characterized in the liver by thin layer chromatography, radio-gas chromatography and mass spectrometric analysis. Four metabolites, resulting from the oxidation of the alkyl chain or of the cyclohexane ring were identified; namely, 3-dodecylcyclohexanol, 4-dodecylcyclohexanol, cyclohexyldodecane-2-ol, and cyclohexyldodecanoic acid. This latter metabolite accounted for 30% of the liver radioactivity 24 hr after dosing. The toxicological relevance of this pathway is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01059969
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