ISSN:
1573-0603
Keywords:
bronchial epithelium
;
cell culture
;
intermediate filaments
;
carbohydrate cytochemistry
;
lectins
;
peroxidase-labeled lectins
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A protocol is presented for correlating the morphology of human bronchial epithelial cells after Papanicolaou staining with their periodic acid schiff (PAS)/AB-reactivity, intermediate filament type, and the pattern of staining with lectins having specific affinities for glycoconjugates:griffionia simplicifolia (GSA-IB4; terminal alpha-d-galactose),Dolichos biflorus (DBA,N-acetyl-d-galactosamine). The combination of these stains identified differentiation markers in morphologic studies of normal, transformed, and malignant bronchial epithelial cells in culture.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02388197
Permalink