ISSN:
1573-8264
Keywords:
apoplast - cytoplasm
;
14C-glucose
;
14C-sucrose
;
sugar localization
;
vacuole
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The influence of phosphate deficiency on the sugar accumulation and sugar partitioning in the root cells of bean (Phaseolus vulgaris L.) was studied. Bean plants were cultured 17 - 19 d on a phosphate-sufficient and phosphate-deficient nutrient medium. Phosphate deficit in the growth medium resulted in increased sugar concentration for about 30 % in the apoplastic and cytoplasmic compartments as well as in the vacuoles of root cells. However, the distribution of sugars between apoplast and cytoplasm compartment and vacuole was not affected by decreased phosphate concentration. About 20 % of sugars were found in the apoplast and cytoplasm, about 80 % in the vacuole. Low phosphate concentration enhanced influx of exogenous 14C-sucrose into meristematic and elongation zones of root. The 14C-labelled sugar content in the root tips increased for about 60 % as compared to control plants. Phosphate deficiency increased also 14C-glucose uptake and content in the root tips. However, the amount of 14CO2 liberated during respiration of P-deficient roots (after feeding with uniformly labelled 14C-glucose) was lower than 14CO2 respired by control plants, thus a large part of accumulated sugars seems to be metabolically inactive.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1002108601862
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