Publication Date:
2014-01-01
Description:
Flavonols are the most abundant of all the flavonoids and play pivotal roles in a variety of plants. We isolated a cDNA clone encoding flavonol synthase fromScutellaria baicalensis(SbFLS). The SbFLS cDNA is 1011 bp long, encodes 336 amino acid residues, and belongs to a family of 2-oxoglutarate-dependent dioxygenases. The overall structure ofSbFLSis very similar to that ofArabidopsis thalianaanthocyanidin synthase (AtANS), with aβjelly-roll fold surrounded by tens of short and longα-helices.SbFLSwas constitutively expressed in the roots, stems, leaves, and flowers, with particularly high expression in the roots and flowers. SbFLS transcript levels in the roots were 376-, 70-, and 2.5-fold higher than in the leaves, stems, and flowers. The myricetin content was significantly higher than that of kaempferol and quercetin. Therefore, we suggest that SbFLS mediates flavonol formation in the different organs ofS. baicalensis. Our study may contribute to the knowledge of the role of FLS inS. baicalensis.
Print ISSN:
2356-6140
Electronic ISSN:
1537-744X
Topics:
Natural Sciences in General
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