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  • 1
    Electronic Resource
    Electronic Resource
    Immunohistochemical localization of ascorbate oxidase in cucurbita pepo medullosa
    Amsterdam : Elsevier
    Plant Science 64 (1989), S. 61-66 
    Details
    ISSN: 0168-9452
    Keywords: Cucurbita pepo medullosa ; ascorbate oxidase ; immunohistochemistry
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(89)90152-0
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  • 2
    Electronic Resource
    Electronic Resource
    Intra- and interspecific reproductive barriers inCrocus (Iridaceae) (1996)
    Springer
    Plant systematics and evolution 201 (1996), S. 83-92 
    Details
    ISSN: 1615-6110
    Keywords: Iridaceae ; Crocus ; Pistil transmitting tract ; intra-interspecific pollination ; pregamic-postgamic barriers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The reproductive system was studied inCrocus genus (Iridaceae) following intra- and interspecific pollination, by using light and scanning electron microscopy. The results suggest that the stigma-style tract of theCrocus pistil is a mere promoter of pollen tube growth, while intra- and interspecific discrimination of compatible and incompatible pollen occurs in the ovarian tract. Here, the transmitting tissue consists of special epidermal cells, whose granular or floccular secretions provide the selective medium for the growth of pollen tubes. The ovarian self-incompatibility (SI) is widespread within the genus, resulting in a partial or complete suppression of self-fertilization. Moreover, postzygotic SI mechanisms, as well as postzygotic mechanisms of unknown nature, seem to be recurrent and both are responsible for seed abortion. The interspecific ovarian incompatibility concerns only unrelated crosses; crosses between related fertile species succeed both in fertilization and seed-set.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00989053
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  • 3
    Electronic Resource
    Electronic Resource
    Pregamic and postgamic self-incompatibility systems inCrocus (Iridaceae) (1993)
    Springer
    Plant systematics and evolution 185 (1993), S. 219-227 
    Details
    ISSN: 1615-6110
    Keywords: Iridaceae ; Crocus thomasii ; Crocus vernus subsp.vernus ; Self- ; outcross- ; mixed- ; double-pollination ; fertilization ; seed set ; embryo-endosperm abortion ; self-incompatibility mechanisms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ovule fertilization and seed set were investigated inCrocus thomasii andCrocus vernus subsp.vernus, after different matings. The results gave evidence for a strict ovarian barrier inC. thomasii, which prevents self-fertilization either in self- or mixed- and double-pollination. InC. vernus the ovarian barrier to selfing is not so effective, but a further incompatibility reaction is expressed after self-fertilization resulting in a complete failure of embryogenesis. The implications of the present observations in respect to the mechanisms of pregamic- and postgamic rejection, indicate that a gametophytic system of self-incompatibility controls the ovule fertilization, and a not yet defined system of self-incompatibility controls embryogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00937659
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  • 4
    Electronic Resource
    Electronic Resource
    Embryology ofCrocus thomasii (Iridaceae) (1989)
    Springer
    Plant systematics and evolution 168 (1989), S. 39-47 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Iridaceae ; Crocus thomasii ; Embryology ; intrageneric relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The embryology ofCrocus thomasii is described. Male meiosis is of simultaneous type, and gives rise to starchy microspores which develop into lipoid pollen grains; these are two-celled and show a spinulate acolpate, abaculate exine lacking apertures. The tapetum is glandular and its cells become bi- or sometimes multinucleate. The ovule is anatropous and bitegmic; the inner integument forms the micropyle. Megasporogenesis is heteropolar with starch accumulation in the functional chalazal megaspore. Embryo sac development conforms to thePolygonum type. The endosperm development is nuclear. The embryo develops according to the Caryophyllad type. In the ripe seed it is differentiated and enveloped by a starchy cellular endosperm. The embryological characters observed strongly favour a close relation betweenC. thomasii andC. sativus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00936105
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  • 5
    Electronic Resource
    Electronic Resource
    Self-incompatibility inCrocus vernus subsp.vernus (Iridaceae) (1990)
    Springer
    Plant systematics and evolution 172 (1990), S. 77-82 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Iridaceae ; Crocus vernus subsp.vernus ; Embryology ; mentor effect ; pollen tube ; self-incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Outcross, self- and mixed pollinations were performed inCrocus vernus subsp.vernus, a species with bicellular pollen, dry stigmas and hollow style. No differences were noted among the above pollinations concerning the germination of pollen and the growth of pollen tubes until the top of ovary. Within 45 min after pollinations 62% of pollen grains germinated. Pollen tubes penetrated the papilla cuticle extending along the papilla wall; on entry into stigmatic lobes they continued growth in the stylar secretion to ovarian locules. Here, however, self-pollen tubes failed to reach or to enter the ovule micropyle; while pollen tubes from either outcross- or mixed pollinations grew until fertilizing ovules. These observations gave evidence of a self-incompatibility system inCrocus, which appeared to be neutralized by mentor effect. The ovary as site of incompatibility response is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00937799
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  • 6
    Electronic Resource
    Electronic Resource
    In vitro development of parthenocarpic fruits of Crocus sativus L. (1987)
    Springer
    Plant cell, tissue and organ culture 11 (1987), S. 75-78 
    Details
    ISSN: 1573-5044
    Keywords: Crocus sativus L. fruit development in vitro ; parthenocarpy ; 2,4-dichlorophenoxyacetic acid ; benzyladenine ; gibberellin ; abscisic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In vitro development of parthenocarpic fruits of Crocus sativus L. was induced by culturing ovaries on MS agar medium supplemented with growth-regulators (2,4-D, GA3 and BAP). Amongh these, 2,4-D was the most effective in promoting fructification. The fructigenic activity was independent of both the stage at which the ovaries were excised (before, during or after anthesis) and pollination of the stigmas. Unlike the above compounds, abscisic acid inhibited fructification.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00036578
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