ISSN:
1432-072X
Keywords:
Key words Outer membrane protein
;
Porin
;
Extremophile
;
Piezophile
;
Barophile
;
High pressure adaption
;
Deep-sea bacterium
;
Photobacterium
;
Gene replacement
;
Transposon
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract OmpH is an outer membrane protein produced by the deep-sea bacterium Photobacterium species strain SS9 in response to elevated hydrostatic pressure. In order to facilitate studies of the function of this protein, a series of OmpH+ and OmpH– strains were obtained from SS9 by Tn5 gene replacement mutagenesis. A previously isolated ompH:: lacZ strain and a derivative of this strain harboring a plasmid expressing the wild-type ompH gene were also utilized. The acridine mutagen ICR 191 preferentially inhibited the growth of OmpH+ over OmpH– cells. Indeed, OmpH+ cultures treated with the mutagen rapidly accumulated mutants producing reduced levels of OmpH. In addition, OmpH+ cells took up the peptide Met-Leu-Phe approximately 15 times more rapidly than OmpH– cells. The results are consistent with the hypothesis that OmpH functions as a relatively large, nonspecific diffusion channel.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002030050145
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