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  • 1
    Publication Date: 2021-02-01
    Description: Organic farming systems are gaining popularity as agronomically and environmentally sound soil management strategies with potential to enhance soil microbial diversity and fertility, environmental quality and sustainable crop production. This work aimed at understanding the effect of organic and conventional farming on the diversity of soybean nodulating bradyrhizobia species. Field trapping of indigenous soybean Bradyrhizobium was done by planting promiscuous soybeans varieties SB16 and SC squire as well as non-promiscuous Gazelle in three organic and three conventional farms in Tharaka-Nithi County of Kenya. After 45 days of growth, 108 nodule isolates were obtained from the soybean nodules and placed into 13 groups based on their morphological characteristics. Genetic diversity was done by polymerase chain reaction (PCR) targeting 16S rDNA gene using universal primers P5-R and P3-F and sequencing was carried out using the same primer. High morphological and genetic diversity of the nodule isolates was observed in organic farms as opposed to conventional farms. There was little or no genetic differentiation between the nodule isolates from the different farms with the highest molecular variation (91.12%) being partitioned within populations as opposed to among populations (8.88%). All the isolates were identified as bradyrhizobia with close evolutionary ties with Bradyrhizobium japonicum and Bradyrhizobium yuanminense. Organic farming systems favor the proliferation of bradyrhizobia species and therefore a suitable environmentally friendly alternative for enhancing soybean production.
    Electronic ISSN: 2571-581X
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Frontiers Media
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  • 2
    Publication Date: 2019-08-25
    Description: Sixteen progeny lines of common beans obtained from single crosses made between two parents, GLP2 and KAT B1, were grown in randomized complete block design in a rainout shelter at the Agricultural and Mechanization Research Institute, Machakos, Kenya. The experiment was conducted to study inheritance of traits associated with drought stress adaptation and to establish if significant variation for those traits was existing in order to carry out selection for drought tolerance. The calculated mean values were used to estimate heritability, genetic advance, and correlation study for each trait. Water stress had a significant (p≤0.01) effect on the number of pods per plant, grains per plant, 100-seed weight, and yield per plant. The highest values for genotypic coefficient of variation (36.11%) and phenotypic coefficient of variation (36.70%) were recorded for pods plant-1 under stress condition. Highest broad-sense heritability estimates (96.54%, 94.97%, and 93.16%) coupled with high genetic advance as percent of the mean (22.32%, 34.97%, and 26.32%) were obtained for the number of pods plant−1, days to maturity, and yield plant−1, respectively, showing that selection of these traits together could lead to yield improvement under stressed conditions. Harvest index showed a significant and positive relationship with biomass aboveground (r=0.86) and the number of pods plant−1 (r=0.86) indicating the possibility of identifying high performing lines of common beans for drought stress environment for further studies on these traits.
    Print ISSN: 1687-8159
    Electronic ISSN: 1687-8167
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Hindawi
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