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  • 1
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis from mature embryo-derived leaflets of peanut (Arachis Hypogaea L.) (1994)
    Springer
    Plant cell reports 13 (1994), S. 578-581 
    Details
    ISSN: 1432-203X
    Keywords: Mature embryo derived ; Conversion ; Arachis hypogaea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Embryogenic masses were obtained from immature leaves of peanut (Arachis hypogaea L.) cultured on a medium containing 20 mg/l 2,4-D. Somatic embryos developed from these masses following transfer to a medium containing 3 mg/l 2,4-D. The embryo morphology was quite variable. Following transfer to hormone-free medium, these embryos germinated. Shoot elongation was obtained in 25% of the embryos following transfer to a medium supplemented with 0.5 mg/l each of BAP and Kn. The plants grown in vitro by this method survived in sand:soil mixture and were grown to maturity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234515
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  • 2
    Electronic Resource
    Electronic Resource
    High-frequency conversion of abnormal peanut somatic embryos (1997)
    Springer
    Plant cell reports 16 (1997), S. 783-786 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsArachis hypogaea ; Abnormal somatic embryos ; Conversion ; Thidiazuron
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Peanuts (Arachis hypogaea L.) are widely cultivated as a rich source of protein and oil. Although protocols for the regeneration of peanut via somatic embryogenesis and organogenesis have been developed, most of them have resulted in low frequencies of plant recovery. In this report, we describe a protocol for plantlet formation at high frequency from somatic embryos. Morphologically abnormal somatic embryos germinated and produced roots only in medium devoid of growth regulators. Shoots emerged from the undeveloped plumule of these rooted embryos in medium containing both 6-benzyladenine (BA) and kinetin (KN), or in medium with thidiazuron (TDZ) alone. In Murashige and Skoog basal medium supplemented with 8.9 µm BA and 14 µm KN, 86% of the embryos developed shoots. Substitution of BA and KN with 22.7 µm TDZ increased plant recovery from 86% to 92%. Plants grown on TDZ had multiple shoots. Eighty-four percent of these plants survived in sandy soil and were grown to maturity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050320
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  • 3
    Electronic Resource
    Electronic Resource
    Influence of osmotica and abscisic acid on triglyceride accumulation in peanut somatic embryos (1998)
    Springer
    Plant cell reports 17 (1998), S. 742-746 
    Details
    ISSN: 1432-203X
    Keywords: Key words Sucrose ; Mannitol ; Sorbitol ; Osmolality ; Abscisic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Attempts were made to determine the influence of sucrose, mannitol, sorbitol and abscisic acid on accumulation of triglycerides in peanut (Arachis hypogaea L.) somatic embryos. The results revealed that 0.584 m sucrose in the medium produced increased triglycerides in the embryos compared to the control. At 0.730 m sucrose, embryos were necrotic although the triglyceride content was high. Sorbitol at 0.6 m or abscisic acid at 20 μm were effective in increasing triglycerides in the embryos. The increase in triglycerides on a fresh-weight basis observed with increasing concentration of osmoticium was not apparent when determined in terms of dry weight. However, an increase in triglyceride as percent fresh weight observed in the presence of 20 μm abscisic acid remained unaltered when determined in terms of percent dry weight. An increase in storage lipid did not improve conversion of peanut somatic embryos.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050476
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  • 4
    Electronic Resource
    Electronic Resource
    Genotypic control of peanut somatic embryogenesis (1998)
    Springer
    Plant cell reports 17 (1998), S. 522-525 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsArachis hypogaea L. ; Somatic embryogenesis ; Genotypes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The protocol for obtaining a high frequency of plant development via somatic embryogenesis from mature zygotic embryo-derived leaflets of peanut (Arachis hypogaea L.) involves multiple stages; these include the induction of embryogenic masses, development of embryos, radicle emergence/conversion of embryos and the development of plants from rooted abnormal embryos. Sixteen genotypes were subjected to this protocol by exposing mature zygotic embryo-derived leaflets to the common media sequence and comparing responses. Although the protocol was effective for all the genotypes, variation in frequency of response at each stage of development indicated that, with the exception of root meristem differentiation and subsequent radicle emergence, the whole process of somatic embryogenesis depended on the genotypic constitution of the original plant. The failure of somatic embryos to undergo conversion to plantlets could be a genotype-dependent characteristic.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050435
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