ISSN:
1365-2958
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
,
Medicine
Notes:
To determine whether the RNA of bacterial viruses is polyadenylated like bacterial mRNAs, pulse-labelled as well as the steady-state population of bacteriophage T7-specific transcripts were examined for the presence of poly(A) tracts by binding to oligo(dT) cellulose followed by hybridization with specific gene probes. Representatives of all classes of bacteriophage-specific mRNA — early, middle and late — were found to be polyadenylated. This conclusion was confirmed by screening the products of oligo(dT)-dependent cDNA synthesis. A cDNA library was prepared from RNA synthesized after bacteriophage T7 infection and the sequence of bacteriophage-specific clones was determined to define the sites of polyadenylation. About half of the clones were polyadenylated near the end of a protein-coding region, one of them at the site of post-transcriptional processing by RNase III. Other clones were polyadenylated within protein-coding regions. These observations suggest that polyadenylation occurs after the nucleolytic processing of primary transcripts and in some cases also after mRNA degradation has already begun.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1046/j.1365-2958.1998.00649.x
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