ISSN:
1615-6102
Keywords:
Legumes
;
Nitrogen fixation
;
Nodulation
;
Rhizobium
;
Symbiosis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Two methods have been developed in order to discriminate between lateral roots, nodules and root-derived structures which exhibit both root and nodule histological features and which can develop on legumes inoculated with certainRhizobium mutants. The first method, known as the “clearing method”, allows the observation by light microscopy of cleared undissected root-structures. The second, known as the “slicing method”, is a complementary technique which provides a greater degree of structural information concerning such structures. The two methods have proved invaluable in defining unequivocally the nature of the interaction between a rhizobial strain and a legume host.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01322980
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